Improved in vitro method for screening organophosphate-induced delayed polyneuropathy

Toxicology in Vitro : an International Journal Published in Association with BIBRA
G Schmuck, H J Ahr

Abstract

Organophosphate-induced delayed polyneuropathy (OPIDP) has been tested mainly byin vivo methods in the chicken and by examination of the inhibition of neuropathy target esterase in their neuronal tissue. An alternative method, using permanent neuronal cell lines and detecting the growth of neurite-like sprouts by morphometric means, has meanwhile been validated to substitute thesein vivo investigations, at least in part. This paper reports some modifications of this in vitro method in order to optimize it for a routine laboratory screening test of organophosphates. By determination of cytoskeletal elements with cell ELISA, it was demonstrated that seven OPIDP-inducing compounds [tri-o-cresyl phosphate (TOCP) and its metabolite cresyl-saligeninphosphate, haloxon, mipafox, leptophos, EPN and chlorpyrifos] and nine negative control substances [paraoxon, methylazinphos, ethylazinphos, dimefox, dimethoate, phenylmethanesulfonylfluoride (PMSF) and three organophosphate metabolite-cresol, phenol and p-nitrophenol] resulted in the same effects as seen in the original test method. The cell ELISA technique thus represents an alternative method that is much easier to perform than the morphometric method.

References

Jul 1, 1979·Journal of Neurochemistry·M L Shelanski, R K Liem
Jan 1, 1991·Critical Reviews in Toxicology·M Lotti
Mar 1, 1990·Toxicology and Applied Pharmacology·M K Johnson
Jan 1, 1990·Annual Review of Pharmacology and Toxicology·M B Abou-Donia, D M Lapadula
Jul 26, 1968·Science·P BendaW Sweet
Sep 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·G Augusti-Tocco, G Sato
Jan 1, 1981·Annual Review of Pharmacology and Toxicology·M B Abou-Donia
May 1, 1995·Clinical and Experimental Pharmacology & Physiology·M B Abou-Donia
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE
Jul 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D HenschlerD Schiffmann

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Citations

Dec 28, 1999·Brain Research. Developmental Brain Research·K DamT A Slotkin
Sep 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M SachanaA J Hargreaves
Jan 23, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M SachanaA J Hargreaves
Apr 18, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M SachanaA J Hargreaves
Feb 1, 2006·Environmental Toxicology and Pharmacology·Sandra CoeckeThomas Hartung
Jan 1, 2005·Toxicology Mechanisms and Methods·Magdalini SachanaAlan J Hargreaves
Dec 21, 2010·Toxicology Letters·Erasmia SidiropoulouZerai Woldehiwet
Oct 1, 2005·Alternatives to Laboratory Animals : ATLA·Pilar PrietoCarl Westmoreland
Jul 13, 2006·Alternatives to Laboratory Animals : ATLA·Pilar PrietoWalter Pfaller

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