Improved intracellular delivery of peptide- and lipid-nanoplexes by natural glycosides

Journal of Controlled Release : Official Journal of the Controlled Release Society
Alexander WengStephen L Hart

Abstract

Targeted nanocarriers undergo endocytosis upon binding to their membrane receptors and are transported into cellular compartments such as late endosomes and lysosomes. In gene delivery the genetic material has to escape from the cellular compartments into the cytosol. The process of endosomal escape is one of the most critical steps for successful gene delivery. For this reason synthetic lipids with fusogenic properties such as 2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) are integrated into the nanocarriers. In this study we show that a natural, plant derived glycoside (SO1861) from Saponaria officinalis L. greatly improves the efficacy of lipid based as well as non-lipid based targeted nanoplexes consisting of a targeted K16 peptide with a nucleic acid binding domain and plasmid-DNA, minicircle-DNA or small interfering RNA (siRNA). By confocal live cell imaging and single cell analyses, we demonstrate that SO1861 augments the escape of the genetic cargo out of the intracellular compartments into the cytosol. Co-localisation experiments with fluorescence labelled dextran and transferrin indicate that SO1861 induces the release of the genetic cargo out of endosomes and lysosomes. However, the transduction efficacy of a le...Continue Reading

References

Jan 30, 1991·Biochimica Et Biophysica Acta·R C MacDonaldL R Hu
Jan 1, 1994·American Journal of Respiratory Cell and Molecular Biology·A L CozensD C Gruenert
May 4, 1995·Biochimica Et Biophysica Acta·H FarhoodL Huang
Dec 28, 1999·Protein Engineering·P Corish, C Tyler-Smith
Aug 29, 2003·The Journal of Biological Chemistry·N D SonawaneA S Verkman
Dec 11, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dasein Pinto-González HowellMichael A Barry
Sep 10, 2004·Molecular Biology of the Cell·Vilja PietiäinenTimo Hyypiä
Aug 2, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·James HeyesIan MacLachlan
Oct 6, 2005·Annals of Medicine·Vilja M PietiäinenTimo Hyypiä
Nov 18, 2005·Gene Therapy·J L Anderson, T J Hope
Nov 25, 2005·Current Drug Delivery·Stephen L Hart
Aug 18, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Luc Wasungu, Dick Hoekstra
Jan 20, 2007·Biochemical Society Transactions·D HoekstraI Zuhorn
Sep 4, 2008·The Journal of Gene Medicine·Peter MayrhoferWolfgang Jechlinger
Apr 23, 2009·Advanced Drug Delivery Reviews·Tamaki Endoh, Takashi Ohtsuki
May 7, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Conrad A VinkWaseem Qasim
Jun 6, 2009·Experimental Biology and Medicine·Alexander WengMatthias F Melzig
Jan 12, 2010·Journal of Internal Medicine·A SchroederD G Anderson
Feb 11, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Alexander WengMatthias F Melzig
Mar 6, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stephanie M GrosseStephen L Hart
Apr 2, 2010·Journal of Cell Science·Monika Dominska, Derek M Dykxhoorn
Nov 17, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Amir K VarkouhiHidde J Haisma
Nov 26, 2010·Nature Biotechnology·Mark A KayZhi-Ying Chen
May 3, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Amir Khashayar VarkouhiGert Storm
Jun 1, 2011·Biomaterials·Aristides D TagalakisStephen L Hart
May 26, 2012·Biochimica Et Biophysica Acta·Daniela PozziGiulio Caracciolo
Jun 6, 2012·Molecular Pharmaceutics·Kyung-mi ChoiHyung Jun Ahn
Aug 10, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Lorena Redondo-MorataFausto Sanz
Sep 15, 2012·Chemical Biology & Drug Design·Matt GoodingDavid L Selwood
Oct 16, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alexander WengHendrik Fuchs
Jan 25, 2013·Molecular Therapy. Nucleic Acids·Nathan M BelliveauPieter R Cullis
Apr 17, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ayman El-Sayed, Hideyoshi Harashima
May 15, 2013·FEBS Letters·Chérine Bechara, Sandrine Sagan
Jul 28, 2013·International Journal of Biological Macromolecules·Mayank ThakurHendrik Fuchs
Oct 3, 2013·Bioorganic & Medicinal Chemistry·Stefan Böttger, Matthias F Melzig

❮ Previous
Next ❯

Citations

Apr 17, 2016·Nucleic Acids Research·Rudolph L Juliano
Feb 17, 2018·Journal of Translational Medicine·Pan WuChunmao Han
Jun 12, 2021·Acta Ophthalmologica·Amisha SanghaniCynthia Yu-Wai-Man

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.