Improved isolation strategies allowed the phenotypic differentiation of two Nitrospira strains from widespread phylogenetic lineages

FEMS Microbiology Ecology
Boris NowkaEva Spieck

Abstract

The second step of nitrification, the oxidation of nitrite to nitrate, is vital for the functioning of the nitrogen cycle, but our understanding of the ecological roles of the involved microorganisms is still limited. The known diversity of Nitrospira, the most widely distributed nitrite-oxidizing bacteria, has increased remarkably by analyses of 16S rRNA and functional gene sequences. However, only few representatives could be brought into laboratory cultures so far. In this study, two Nitrospira from activated sludge were isolated using novel approaches together with established methods. Highly enriched 'Candidatus Nitrospira defluvii' was separated from concomitant heterotrophs by taking advantage of its resistance against ampicillin and acriflavine. Beside this member of lineage I, a novel species of lineage II, named N. lenta, was initially enriched at 10°C and finally purified by using optical tweezers. The tolerance to elevated nitrite levels was much higher in N. defluvii than in the more fastidious N. lenta and was accompanied by pronounced biofilm formation. Phylogenetic classification of 12 additional enrichments indicated that Nitrospira lineage I is common in extreme and moderate ecosystems like lineage II. The new...Continue Reading

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Citations

Aug 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Hanna KochHolger Daims
Nov 27, 2015·Nature·Holger DaimsMichael Wagner
Jan 27, 2017·Environmental Science & Technology·Mee-Rye ParkKartik Chandran
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Apr 15, 2020·Environmental Microbiology·Kento IshiiSatoshi Tsuneda
Nov 12, 2018·Applied Microbiology and Biotechnology·Hanna KochSebastian Lücker
Aug 28, 2020·Frontiers in Microbiology·Yu TakahashiSatoshi Tsuneda
Jan 16, 2021·Journal of Basic Microbiology·Ardhra VijayanBright Singh Isaac Sarojini
Sep 12, 2021·Applied Microbiology and Biotechnology·Eva SpieckSabine Keuter

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