PMID: 8972874Dec 1, 1996Paper

Improved large scale culture of Methylophilus methylotrophus for 13C/15N labeling and random fractional deuteration of ribonucleotides

Nucleic Acids Research
R T BateyJ R Williamson

Abstract

Isotopic labeling of RNA with 13C and 15N has become a routine procedure in structural studies by NMR spectroscopy. The methodology in this paper describes the random fractional deuteration of RNA using the obligate methylotropic bacterium, Methylophilus methylotrophus. This bacterium was grown using a non-deuterated carbon source in 52:48 D20/H20 and we have shown that all protons in the ribonucleotides except for the ribose H1 become 52% randomly fractionally deuterated. Improved growth conditions for this organism are also described that yield higher cell densities in liquid culture, which is applicable for all labeling procedures.

References

Jan 15, 1960·Biochimica Et Biophysica Acta·W CHORNEYJ J KATZ

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Citations

Nov 18, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A E Tomkinson, D S Levin
Jun 15, 2010·Journal of Biomolecular NMR·Christian RichterHarald Schwalbe
Nov 5, 1997·Current Opinion in Structural Biology·L E Kay, K H Gardner
Dec 21, 2010·Journal of the American Chemical Society·Heather L SchultheiszJames R Williamson
Apr 12, 2012·Journal of the American Chemical Society·Christoph H WunderlichChristoph Kreutz
Jul 1, 1998·Annual Review of Biophysics and Biomolecular Structure·K H Gardner, L E Kay
Mar 28, 2009·International Journal of Molecular Sciences·Kwaku T Dayie
Jul 8, 2017·The Science of the Total Environment·Karla Martinez-CruzMary Beth Leigh
Jul 31, 2021·ACS Chemical Biology·Dale A CummingsAaron W Puri
Jul 20, 2006·Journal of the American Chemical Society·Pramodh VallurupalliLewis E Kay
May 5, 2005·Journal of the American Chemical Society·Pramodh Vallurupalli, Lewis E Kay

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