Improved liquid chromatographic method with pulsed electrochemical detection for the analysis of gentamicin

Journal of Chromatography. a
Vicky ManyangaErwin Adams

Abstract

The official method for the determination of the composition and related substances of gentamicin prescribed by the European Pharmacopoeia (Ph. Eur.) is liquid chromatography combined with pulsed electrochemical detection (LC-PED). However, this method utilizes a polymer stationary phase which shows rather low efficiency towards the separation of the main gentamicin components. Moreover, the mobile phase contains a lot of non volatile salts: sodium sulphate and sodium octanesulphonate. Following a comparative study, the most performant LC-PED method has been evaluated and validated using a reversed phase C18 column (C18, 250 x 4.6mm ID, 110 A, 5 microm) kept at 35 degrees C with a mobile phase containing volatile ion pairing agents: trifluoroacetic acetic acid (TFA) and pentafluoropropionic acid (PFPA). In addition to the selectivity of the main gentamicin components and its related substances, the method is repeatable, linear and proves to be robust. It is also applicable to a wider number of C18 columns.

Citations

Oct 29, 2010·Journal of Automated Methods & Management in Chemistry·Lúcia H M L M SantosM C B S M Montenegro
Feb 15, 2011·Journal of Pharmaceutical and Biomedical Analysis·Bo LiErwin Adams
Mar 30, 2010·Journal of Pharmaceutical and Biomedical Analysis·Shruti ChopraErwin Adams
Nov 6, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xin LuShulin Zhao
Jul 7, 2009·Journal of Pharmaceutical and Biomedical Analysis·Rok Grahek, Lucija Zupancic-Kralj
May 12, 2009·Journal of Pharmaceutical and Biomedical Analysis·Susan D Van ArnumChinfong Chang
May 8, 2016·Talanta·Oliver Wahl, U Holzgrabe
Nov 7, 2015·SpringerPlus·Jim C E OdekerkenTim J M Welting
Oct 22, 2008·Critical Reviews in Biotechnology·C Ganesh KumarAnnapurna Jetty
Mar 14, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tuo MengQingguo Xu
Aug 4, 2009·Journal of Agricultural and Food Chemistry·Xiangmei LiXilong Xiao

Related Concepts

2-deoxystreptamine hydrochloride
Perfluoropropionic acid, sodium salt
Liquid Chromatography
Electrochemistry
Telomer Fluorocarbons
Gentacycol
Hexosamines
Siseptin
Trifluoroacetate

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