PMID: 8601698Apr 1, 1996Paper

Improved localization of fluorescent tyramides for fluorescence in situ hybridization using dextran sulfate and polyvinyl alcohol

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
R P van GijlswijkH J Tanke

Abstract

Recently, a peroxidase-mediated amplification system has been described for immunofluorescence and fluorescence in situ hybridization studies. It is based on the deposition of hapten- or fluorochrome-labeled tyramide molecules. Although providing a significantly increased detection sensitivity compared to conventional procedures, its localization properties are inferior because of free diffusion of intermediate reaction products before they are immobilized. In enzyme cytochemistry, it is well established that improved localization of enzyme activity can be achieved through the addition of viscosity-increasing polymers to the incubation media. In this study we analyzed the effect of different polymers on the localization sharpness and sensitivity of the tyramide-peroxidase reaction in FISH applications. Significantly improved localization of the fluorescent endproduct was observed using dextran sulfate or polyvinylalcohol (PVA) with, respectively, no or little loss of sensitivity.

Citations

Aug 1, 1996·Histochemistry and Cell Biology·R W Dirks
Jun 24, 1999·Journal of Neuroscience Methods·A StanariusG Wolf
Sep 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Akio KatoJames A Birchler
Dec 10, 2009·World Journal of Gastroenterology : WJG·Christina LohwasserDetlef Schuppan
Jul 5, 2007·Molecular Genetics and Genomics : MGG·Alexa Telgmann-RauberChristian Jung
Sep 7, 2004·Expert Review of Molecular Diagnostics·Kathleen DorritieThomas Ried
Feb 24, 2001·Progress in Histochemistry and Cytochemistry·G Mayer, M Bendayan
Mar 26, 2011·Journal of Clinical Laboratory Analysis·Thomas S LawsonJames A Piper
May 24, 2011·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Ryo NasgashioMakoto Saegusa
Mar 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P T MacechkoS L Erlandsen
Mar 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R P van GijlswijkA K Raap
Nov 14, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Mayer, M Bendayan
Nov 14, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R von WasielewskiA Georgii
Oct 6, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E J SpeelA H Hopman
Mar 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Mayer, M Bendayan
Feb 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E J SpeelP Komminoth
Jun 17, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A KöhlerC J Van Noorden
Jan 29, 2016·Methods : a Companion to Methods in Enzymology·Giselbert HauptmannIris Söll
Sep 5, 2008·Current Protocols in Cytometry·J Wiegant
Sep 5, 2008·Current Protocols in Cytometry·M N Bobrow, P T Moen
Oct 6, 2004·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Erik J OlsonArno Wünschmann
Jul 2, 2020·The Journal of Cell Biology·Joseph DopieAndrew S Belmont
Mar 29, 2020·PeerJ·Alexander P YoungRussell C Wyeth
Feb 1, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A BükiJ T Povlishock
Sep 14, 2017·Journal of Proteome Research·Lanyi ChangTakashi Angata

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