Improved long-term durability of allogeneic heart valves in the orthotopic sheep model

European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery
Anna BiermannUlrich A Stock

Abstract

Frozen cryopreservation (FC) with the vapour phase of liquid nitrogen storage (-135°C) is a standard biobank technique to preserve allogeneic heart valves to enable a preferable allograft valve replacement in clinical settings. However, their long-term function is limited by immune responses, inflammation and structural degeneration. Ice-free cryopreserved (IFC) valves with warmer storage possibilities at -80°C showed better matrix preservation and decreased immunological response in preliminary short-term in vivo studies. Our study aimed to assess the prolonged performance of IFC allografts in an orthotopic pulmonary sheep model. FC (n = 6) and IFC (n = 6) allografts were transplanted into juvenile Merino sheep. After 12 months of implantation, functionality testing via 2-dimensional echocardiography and histological analyses was performed. In addition, multiphoton autofluorescence imaging and Raman microspectroscopy analysis were applied to qualitatively and quantitatively assess the matrix integrity of the leaflets. Six animals from the FC group and 5 animals from the IFC group were included in the analysis. Histological explant analysis showed early inflammation in the FC valves, whereas sustainable, fully functional, devit...Continue Reading

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Citations

Sep 11, 2020·Nature Reviews. Cardiology·Emanuela S FiorettaMaximilian Y Emmert
Aug 15, 2020·Expert Review of Cardiovascular Therapy·Nikolaos PoulisMaximilian Y Emmert
Jul 25, 2019·Tissue Engineering. Part C, Methods·Matthias BeckerMartina Seifert
Jan 3, 2019·Analytical Chemistry·Julia MarziKatja Schenke-Layland
Sep 21, 2021·Tissue Engineering. Part C, Methods·Rachel BeattyGarry P Duffy

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