Improved method facilitates reliable APOE genotyping of genomic DNA extracted from formaldehyde-fixed pathology specimens

Journal of Neuroscience Methods
E GhebremedhinJ Sahm

Abstract

Apolipoprotein E (APOE) genotyping of genomic DNA extracted from formaldehyde-fixed specimens is cumbersome: there is not only a low yield or failure of PCR amplification (presumably due to degradation of DNA in the formaldehyde-fixed and paraffin-embedded tissue), but the standard method also involves the separation of DNA fragments as small as 48, 72, 81 and 91 bp requiring high-yield PCR products. Here we report about a semi-nested PCR method suitable for providing specific high-yield PCR products from DNA that has been extracted from formaldehyde-fixed specimens which initially generate low-quality templates. This method facilitates reliable APOE genotyping of DNA from difficult templates.

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Citations

May 5, 2004·Neurobiology of Aging·Christian SchultzHeiko Braak
Feb 8, 2006·Journal of Neuropathology and Experimental Neurology·Estifanos GhebremedhinHeiko Braak
Mar 24, 2012·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Neena Theresa KumarJan Maehlen
Jun 28, 2006·International Journal of Geriatric Psychiatry·Mattias HaglundElisabet Englund
Feb 6, 2020·Journal of Neuropathology and Experimental Neurology·Kelly Del TrediciHeiko Braak
Mar 16, 2002·Journal of Neuropathology and Experimental Neurology·Dietmar Rudolf ThalHeiko Braak
Dec 25, 2003·Journal of Neuropathology and Experimental Neurology·Dietmar Rudolf ThalOtmar D Wiestler
Aug 22, 2000·Journal of Neuropathology and Experimental Neurology·D R ThalH Braak
Feb 8, 2006·Journal of Neuropathology and Experimental Neurology·Barbara SerafiniFrancesca Aloisi
Oct 13, 2021·Acta Neuropathologica Communications·Barbara E StopschinskiMarc I Diamond

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