PMID: 9002073Dec 1, 1996Paper

Improved method for counting virus and virus like particles

Journal of Virological Methods
Y Z ZhengS Reid

Abstract

An improved method for counting virus and virus like particles by electron microscopy (EM) was developed. The procedure involves the determination of the absolute concentration of pure or semi-pure particles once deposited evenly on EM grids using either centrifugation or antibody capture techniques. The counting of particles was done with a Microfiche unit which enlarged approximately 50 x the image of particles on a developed negative film which had been taken at a relatively low magnification (2500 x) by EM. Initially, latex particles of a known concentration were counted using this approach, to prove the accuracy of the technique. The latex particles were deposited evenly on an EM grid using centrifugation (Modified Beckmen EM-90 Airfuge technique). Subsequently, recombinant Bluetongue virus (BTV) core-like particles (CLPs) captured by a Monoclonal antibody using a novel sample loading method were counted by the Microfiche unit method and by a direct EM method. Comparison of the simplified counting method developed with a conventional method, showed good agreement. The method is simple, accurate, rapid, and reproducible when used with either pure particles or with particles from crude cell culture extracts.

References

Aug 10, 1989·Nature·O BerghM Heldal
Jan 1, 1985·Pathology·J JansonsM R Bucens
Nov 1, 1964·Virology·W F ROCHOW, M K BRAKKE
Jan 1, 1949·Proceedings of the Society for Experimental Biology and Medicine·D G SHARP

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Citations

Jun 6, 2008·Journal of Clinical Microbiology·Ion Gutiérrez-AguirreMaja Ravnikar
Sep 19, 2009·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·V V Drucker, N V Dutova
Jan 31, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·X L PangUNKNOWN Canadian Society of Transplantation
Jul 6, 2004·Journal of Virological Methods·Bishnupriya BhattacharyaAriberto Fassati
Jun 21, 2012·Chemistry, an Asian Journal·Pei-Ling ChiangYit-Tsong Chen

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