Improved method for the analysis of alpha-galactosides in pea seeds by capillary zone electrophoresis. Comparison with high-performance liquid chromatography-triple-pulsed amperometric detection

Journal of Chromatography. a
J FriasC Vidal-VALverde

Abstract

A capillary zone electrophoretic (CZE) method is described for the analysis of the raffinose family of oligosaccharides (RFO) in pea seeds. Extraction of RFO was carried out in 80% ethanol and the extract was passed through a Sep-Pak C18 cartridge. This proved to be an improvement on the currently accepted purification technique. High-quality electrophoregrams were obtained which allowed the separation and quantitation of sucrose, raffinose, stachyose and verbascose The CZE results were compared with those obtained by anion-exchange high-performance liquid chromatography coupled to a triple-pulsed amperometric detection (HPAC-PAD). The samples were obtained from four pea strains which were near-isogenic except for genes at the r and rb loci. A high degree of precision and reproducibility was obtained for the RFO compositions of all the pea strains. No statistically significant differences (p < or equal to 0.05) were found between the two analytical techniques using paired Student-t tests.

References

Jan 1, 1988·Die Nahrung·K R PriceG R Fenwick
Nov 1, 1993·Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung·C Vidal-ValverdeJ Bacon

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Citations

Jul 1, 1996·Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung·J FriasC L Hedley
Nov 15, 2014·Critical Reviews in Biotechnology·Irina Yu BakuninaAntonio Trincone
Dec 16, 1998·Electrophoresis·S Suzuki, S Honda
Aug 24, 2000·Journal of Agricultural and Food Chemistry·P GulewiczK Gulewicz

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