Improved methods for the structural analysis of the amylose-rich fraction from rice flour

Biomacromolecules
Rachelle M WardMelissa A Fitzgerald

Abstract

Cooking and sensory properties of rice are largely determined by the amylose content and structure. For relationships between functional and structural properties, a more accurate method to determine the structure of amylose is required. Here we calibrate size exclusion chromatography (SEC) columns, using Mark-Houwink parameters for linear starch and pullulan standards, to obtain the true molecular weight distribution of linear starch. When the molecular weight distribution is reported relative to pullulan, rather than the actual molecular weight which is readily obtained from universal calibration, it is seen that the molecular weights of longer amylose chains are greatly underestimated. We validate the SEC method to enable the measurement of the hydrodynamic volume distribution of the starch by examining reproducibility and recovery. Analysis of the starch in the sample pre- and post-SEC shows that 20% of the carbohydrate is not recovered. Comparison of the weight-average degree of polymerization, X(w), of (undebranched) starch of pre- and post-SEC is made using iodine binding as well as Berry plots of data from multi-angle laser light scattering (MALLS). These both show that current SEC techniques for starch analysis lead to...Continue Reading

References

Jul 1, 1996·International Journal of Biological Macromolecules·P Roger, P Colonna
Aug 26, 1998·The Journal of Biological Chemistry·M van de WalS Ball
Feb 1, 2003·Carbohydrate Research·Yasuhito TakedaIsao Hanashiro

❮ Previous
Next ❯

Citations

May 13, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·N A TranM A Fitzgerald
Nov 26, 2010·Analytical and Bioanalytical Chemistry·Robert G Gilbert
Jul 12, 2013·Analytical and Bioanalytical Chemistry·Robert G GilbertJovin Hasjim
Sep 27, 2012·Journal of Agricultural and Food Chemistry·Vito M ButardoMelissa Fitzgerald
Sep 30, 2014·Carbohydrate Polymers·Alex Chi WuRobert G Gilbert
Jan 6, 2015·Journal of Agricultural and Food Chemistry·Weiwei ChengXiong Fu
Aug 30, 2008·Journal of Chromatography. a·Ngoc-Ly HoangPatrice Castignolles
Sep 26, 2007·Carbohydrate Research·Anthony DonaMarianne Gaborieau
Jan 30, 2007·Journal of Chromatography. a·M L Sanz, I Martínez-Castro
Dec 18, 2008·Macromolecular Bioscience·Sarah SchmitzMarianne Gaborieau
Apr 5, 2013·Phytochemical Analysis : PCA·Somanath AgasimaniSundaram Ganesh Ram
Jan 1, 2010·Functional Plant Biology : FPB·Melissa A Fitzgerald, Adoracion P Resurreccion
Mar 4, 2020·Carbohydrate Polymers·Angela ChemelliStefan Spirk
Feb 21, 2021·Analytica Chimica Acta·Ester LubomirskyR Dario Arrua
Feb 26, 2008·Biomacromolecules·Javier M HernándezRobert G Gilbert
Jan 27, 2019·International Journal of Biological Macromolecules·Hongyan LiBaoguo Sun
May 18, 2021·Data in Brief·Reuben James Q BuenafeNese Sreenivasulu
Dec 30, 2020·Journal of Agricultural and Food Chemistry·Ping-Ping WangTamer Mahmoud Tamer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.