Improved Microassays Used to Test Natural Product-Based and Conventional Fungicides on Plant Pathogenic Fungi

Plant Disease
Maritza AbrilDavid E Wedge

Abstract

Seven important plant pathogenic fungi (Botrytis cinerea, Colletotrichum acutatum, C. fragariae, C. gloeosporioides, Fusarium oxysporum, Phomopsis obscurans, and P. viticola) valuable in screening fungicides were tested. Our procedure included washing conidia to reduce germination times, incorporating Roswell Park Memorial Institute 1640 as a medium of known composition, and using coverslips in the 24-well cell culture clusters to document the effect of fungicides on fungal morphology. The natural product-based fungicide, sampangine, a sampangine analog, 4-bromosampangine, plus seven conventional fungicides (benomyl, captan, cyprodinil, fenbuconazole, fenhexamid, iprodione, and kresoxim-methyl) were tested in vitro for their ability to inhibit germination and growth of the seven fungal species. Sampangine inhibited germination in all fungi except C. acutatum. Comparison of results of germination and morphology microbioassays with results of microtiter assays suggests that some fungicides stop fungal germination, whereas others only slow down fungal growth. We hypothesize that sampangine, except against C. acutatum, has the same physical mode of action, germination inhibition, as the conventional fungicides captan, iprodione, an...Continue Reading

References

Feb 1, 1991·Journal of Clinical Microbiology·A Espinel-Ingroff, T M Kerkering
Jan 6, 2000·Molecular Pathology : MP·R Caldarelli-StefanoG Costanzi
Apr 28, 2000·Phytochemistry·D E WedgeF A Macías
Oct 29, 2002·Pest Management Science·Kumudini M MeepagalaDavid E Wedge
Nov 14, 2002·Journal of Agricultural and Food Chemistry·Kumudini M MeepagalaDavid E Wedge
Feb 6, 2003·Journal of Agricultural and Food Chemistry·Anna OlivaStephen O Duke
Mar 12, 2003·Medical Mycology·S RenaultC P Selitrennikoff

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