Improved O-serotyping method for Serratia marcescens.

Journal of Clinical Microbiology
M A Gaston, T L Pitt

Abstract

In a previous study, we found that some O serotypes of Serratia marcescens, as defined by agglutination tests, were not based on lipopolysaccharide (LPS) O antigens. We developed a dot enzyme immunoassay with a high degree of LPS specificity and tested 104 distinct clinical strains. Only 7 of the 24 existing O antigens were found in more than one strain: O12/O14 (30.8% of strains examined), O21 (12.5%), O8 (8.7%), O6/O7 (5.8%), O4 (3.8%), O18 (2.9%), and O9 (2.9%). Two new antigens, S1254 (13.5%) and S3255 (3.8%), were also found. Agglutination tests with O antisera identified the LPS antigen in only 36 strains. Prodigiosin production was restricted to serotypes O8, O6, and S3255 and strains with a rough or semirough LPS phenotype. Dot immunoassay appears to offer greater accuracy than agglutination tests for serotype identification in S. marcescens.

References

Oct 1, 1979·Journal of Clinical Microbiology·P A GrimontF Pigache
Dec 1, 1989·Journal of Clinical Microbiology·M A Gaston, T L Pitt
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch

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Citations

Oct 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·K ColomR Cisterna
May 1, 1993·Journal of Clinical Microbiology·H M Aucken, T L Pitt
Oct 1, 1991·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·W H Traub
Oct 1, 1991·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·W H Traub

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