Improved operational stability of chloroperoxidase through use of antioxidants

Journal of Biotechnology
Carl Elovson GreyPatrick Adlercreutz

Abstract

Chloroperoxidase (CPO) from Caldariomyces fumago is a potentially very useful enzyme due to its ability to catalyze a large variety of stereoselective oxidation reactions, but poor operational stability is a main limitation for commercial use. In the present study, the possibility of increasing the operational stability by use of antioxidants was investigated using the oxidation of indole as model reaction. Caffeic acid was the antioxidant showing the strongest positive effects, reaching a total turnover number (TTN) of 135,000 at pH 4 and 4 mM hydrogen peroxide, compared to 28,700 in the absence of antioxidant. Portion-wise addition of hydrogen peroxide in the presence of caffeic acid caused a further increase in TTN to 171,000. An alternative way to reach high TTN was to use tert-butyl hydroperoxide as oxidant instead of hydrogen peroxide: a TTN of 600,000 was achieved although the reaction was quite slow. In this case, antioxidants did not have any positive effect. Possible mechanisms for the observed inactivation of CPO are discussed.

Citations

May 25, 2010·Applied Microbiology and Biotechnology·Martin HofrichterTaina Lundell
Jun 12, 2013·Applied Microbiology and Biotechnology·Milja PešićGregorio Alvaro
Feb 12, 2011·Biotechnology Progress·Anna E V HagströmJohn M Woodley
Jun 7, 2014·Chemical Communications : Chem Comm·Dirk HoltmannFrank Hollmann
Aug 15, 2009·Rapid Communications in Mass Spectrometry : RCM·Ulrika TörnvallMartin Hedström
Oct 15, 2009·Angewandte Chemie·Ajda PodgorsekJernej Iskra

Related Concepts

Sodium caffeate
Antioxidant Effect
Caffeic Acids
Catalysis
Chloride Peroxidase
High Pressure Liquid Chromatography Procedure
Hyphomycetes
Enzyme Stability
Yeast Proteins
Oxydol

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