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Improved performance of the PacBio SMRT technology for 16S rDNA sequencing

Journal of Microbiological Methods

Jul 1, 2014

Jennifer J MosherLouis A Kaplan

Abstract

Improved sequencing accuracy was obtained with 16S amplicons from environmental samples and a known pure culture when upgraded Pacific Biosciences (PacBio) hardware and enzymes were used for the single molecule, real-time (SMRT) sequencing platform. The new PacBio RS II system with P4/C...read more

Mentioned in this Paper

Microorganism
NCOR2 wt Allele
Enzymes, antithrombotic
DNA, Ribosomal
Recombinant DNA
Sequencing
Enzymes for Treatment of Wounds and Ulcers
Base Pairing
High-Throughput Nucleotide Sequencing
Enzymes, hematological
7
136
Paper Details
References
  • References2
  • Citations25
1
  • References2
  • Citations25
123

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Improved performance of the PacBio SMRT technology for 16S rDNA sequencing

Journal of Microbiological Methods

Jul 1, 2014

Jennifer J MosherLouis A Kaplan

PMID: 24978594

DOI: 10.1016/j.mimet.2014.06.012

Abstract

Improved sequencing accuracy was obtained with 16S amplicons from environmental samples and a known pure culture when upgraded Pacific Biosciences (PacBio) hardware and enzymes were used for the single molecule, real-time (SMRT) sequencing platform. The new PacBio RS II system with P4/C...read more

Mentioned in this Paper

Microorganism
NCOR2 wt Allele
Enzymes, antithrombotic
DNA, Ribosomal
Recombinant DNA
Sequencing
Enzymes for Treatment of Wounds and Ulcers
Base Pairing
High-Throughput Nucleotide Sequencing
Enzymes, hematological
7
136

Related Papers

Journal of Microbiological Methods

Efficacy of a 3rd generation high-throughput sequencing platform for analyses of 16S rRNA genes from environmental samples

Journal of Microbiological MethodsSeptember 4, 2013
Jennifer J MosherLouis A Kaplan
BMC Bioinformatics

SSPACE-LongRead: scaffolding bacterial draft genomes using long read sequence information

BMC BioinformaticsJune 22, 2014
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Paper Details
References
  • References2
  • Citations25
1
  • References2
  • Citations25
123
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