Abstract
Soluble egg shell membrane protein (SEP) prepared from the egg shell membrane by performic acid oxidation and pepsin digestion was applied to prepare a membrane conjugated with pepsin-solubilized collagen (PSC) by cross-linking with water-soluble carbodiimide. SEP enabled a conjugated membrane to be constructed with a well-developed network structure having thicker collagen fibrils and higher stability than the PSC membrane alone, as indicated by reduced solubility and collagenase-digestion, increased denaturation temperature, and superior flexibility. The SEP-PSC membrane (SEP:PSC of 1:1, w/w) had lower sweatiness than the PSC membrane. Fibroblasts and keratinocytes adhered to the SEP-PSC-coated wells (SEP:PSC of 1:10, w/w) as a fundamental model of an artificial skin similar to that to the PSC-coated wells due to similar expression level of several cell-adhesion proteins. An SEP-PSC coating accelerated the cell growth of fibroblasts, whereas the cell growth of keratinocytes did not change, or decreased slightly due to more rapid differentiation indicated by a more substantial expression of differentiation marker proteins than a PSC-coating without SEP.
References
Jul 1, 1977·Journal of Agricultural and Food Chemistry·K SuyamaS Adachi
Jan 1, 1988·Annual Review of Cell Biology·K BurridgeC Turner
Jan 8, 1981·Biochimica Et Biophysica Acta·G CrombieC Franzblau
May 1, 1982·Burns, Including Thermal Injury·K Maeda, Y Sasaki
Apr 1, 1981·Archives of Biochemistry and Biophysics·R M LeachG P Van Dyke
Jan 1, 1994·Applied Biochemistry and Biotechnology·K SuyamaY Umetsu
Aug 1, 1996·Bioscience, Biotechnology, and Biochemistry·K TakahashiM Hattori
Apr 2, 1999·Journal of Biochemistry·S HattoriS Irie
Jul 10, 1999·Applied Biochemistry and Biotechnology·S IshikawaI Satoh
Nov 28, 2001·American Journal of Clinical Dermatology·Y M BelloW H Eaglstein
Jan 22, 2002·Bioresource Technology·Shin-ichi IshikawaMakoto Itoh
Aug 7, 2002·Bioscience, Biotechnology, and Biochemistry·Koji TakahashiMakoto Hattori
Feb 1, 1962·The Biochemical Journal·J R BAKER, D A BALCH
Oct 1, 1962·Analytical Biochemistry·T BITTER, H M MUIR
Nov 19, 2003·Advanced Drug Delivery Reviews·Zbigniew Ruszczak
May 19, 2004·American Journal of Surgery·H Paul Ehrlich
Sep 30, 2004·Advances in Biochemical Engineering/biotechnology·Kino-oka Masahiro, Masahito Taya
Citations
Jan 31, 2012·Biotechnology Letters·Shinji TakenakaKenji Aoki
May 21, 2011·Cell and Tissue Research·Eri Ohto-FujitaYoriko Atomi
Jul 23, 2013·Food Chemistry·Shuchen HsiehFeng-Huei Lin
Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Mahesh Kumar Sah, Subha Narayan Rath
Jun 10, 2008·Bioscience, Biotechnology, and Biochemistry·Harumi OkadaKoji Takahashi
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Matej BalážRafael Luque