Improved preparation of chick embryonic samples for magnetic resonance microscopy

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
Xiaowei ZhangG A Johnson

Abstract

Previous work demonstrated the power of three-dimensional (3D) magnetic resonance microscopy (MRM) to follow complicated morphologic development in the embryonic cardiovascular system. In this study we describe a new dual-contrast method for specimen preparation that combines perfusion fixation and immersion in fixative with macro- and small molecular gadolinium agents to provide enhanced definition of both the heart wall and chamber. MRM was performed at 9.4 T with image resolutions of 25, 31, and 50 microm isotropic voxels for three stages of chick embryos (day 4, day 5.5, and day 9), and compared to histological sections of the same embryos. The results show considerable improvement of image quality over previous efforts, with better signal-to-noise ratio (SNR) and contrast between the cardiac chamber and myocardial wall. Excellent correlation was shown between the MRM images and histological sections. Thus, 3D high-resolution MRM in combination with the dual-contrast technique is useful for acquiring quantitative 3D morphologic data regarding heart development.

References

Jul 1, 1988·Teratology·E L EffmannG Cofer
Oct 1, 1986·Investigative Radiology·S N BoneM B Thompson
Nov 1, 1996·Computerized Medical Imaging and Graphics : the Official Journal of the Computerized Medical Imaging Society·B R SmithG A Johnson
Mar 26, 1999·Computerized Medical Imaging and Graphics : the Official Journal of the Computerized Medical Imaging Society·B R SmithG A Johnson
Mar 4, 2000·Nature Biotechnology·A Y LouieT J Meade
Feb 17, 2001·Microscopy Research and Technique·B R Smith
Feb 22, 2001·Annual Review of Physiology·D Srivastava
Feb 27, 2001·AJR. American Journal of Roentgenology·R W Sze, K E Yutzey
Jul 4, 2001·Journal of Magnetic Resonance Imaging : JMRI·B HogersR E Poelmann
Feb 28, 2002·Radiology·G Allan JohnsonLaurence W Hedlund
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON
Sep 3, 2001·Angewandte Chemie·Lee JosephsonRalph Weissleder

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Citations

Oct 20, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tim C McQuinnDavid Sedmera
Oct 25, 2013·Journal of Magnetic Resonance Imaging : JMRI·Eric Y ChangChristine B Chung
Jan 18, 2006·Magnetic Resonance Imaging·Richard G SpencerMark P Mattson
Nov 7, 2014·Frontiers in Physiology·William J KowalskiBradley B Keller
Jun 15, 2016·PloS One·Alexander G DyominElena R Gaginskaya
Oct 1, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T Mesud YelbuzMargaret L Kirby
May 24, 2007·Journal of Magnetic Resonance Imaging : JMRI·Alexandra PetietG Allan Johnson
Jan 23, 2006·Optics Express·M W JenkinsA M Rollins
Nov 28, 2008·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·A M DavisJ A Izatt
Oct 21, 2004·Birth Defects Research. Part C, Embryo Today : Reviews·Jan M RuijterAntoon F M Moorman

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