Improved primate foamy virus vectors and packaging constructs

Journal of Virology
Martin HeinkeleinAxel Rethwilm

Abstract

Foamy virus (FV) vectors that have minimal cis-acting sequences and are devoid of residual viral gene expression were constructed and analyzed by using a packaging system based on transient cotransfection of vector and different packaging plasmids. Previous studies indicated (i) that FV gag gene expression requires the presence of the R region of the long terminal repeat and (ii) that RNA from packaging constructs is efficiently incorporated into vector particles. Mutants with changes in major 5' splice donor (SD) site located in the R region identified this sequence element as responsible for regulating gag gene expression by an unidentified mechanism. Replacement of the FV 5' SD with heterologous splice sites enabled expression of the gag and pol genes. The incorporation of nonvector RNA into vector particles could be reduced to barely detectable levels with constructs in which the human immunodeficiency virus 5' SD or an unrelated intron sequence was substituted for the FV 5' untranslated region and in which gag expression and pol expression were separated on two different plasmids. By this strategy, efficient vector transfer was achieved with constructs that have minimal genetic overlap.

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Citations

Jul 13, 2012·Journal of Virology·Kathrin PlochmannCarsten Scheller
Mar 9, 2007·Journal of Virology·Jacqueline Roy, Maxine L Linial
Jul 1, 2011·Journal of Virology·Erik MüllersDirk Lindemann
Aug 8, 2008·Journal of Virology·Annett StangeDirk Lindemann
Dec 17, 2010·Journal of Virology·Jochen BodemAxel Rethwilm
Feb 4, 2011·Journal of Virology·Daniel MatthesAxel Rethwilm
Feb 18, 2011·Journal of Virology·Maximilian J HartlBirgitta M Wöhrl
Mar 28, 2008·Journal of Virology·Melvyn W YapJonathan P Stoye
Feb 14, 2004·Journal of Virology·Thomas JuretzekAxel Rethwilm
Sep 15, 2005·Journal of Virology·Marc CartellieriAxel Rethwilm
May 7, 2013·BMC Developmental Biology·Shahryar KhattakDirk Lindemann
Jul 12, 2005·Retrovirology·Marc CartellieriAxel Rethwilm
Apr 8, 2009·Retrovirology·Kathleen GärtnerCarsten Scheller
Oct 14, 2011·Viruses·Dirk Lindemann, Axel Rethwilm
Oct 14, 2011·Viruses·Tobias MaetzigAxel Schambach
Mar 23, 2013·Viruses·Eun-Gyung LeeMaxine L Linial
Sep 12, 2009·Expert Opinion on Biological Therapy·Grant D Trobridge
Nov 27, 2015·Virology·Ariane BährCarsten Münk
Jul 17, 2008·Traffic·Joelle Tobaly-TapieroAli Saïb
Apr 5, 2013·The Journal of Gene Medicine·Conrad WeberAndre F Steinert
Oct 12, 2012·Cellular Microbiology·Anka SwiersyDirk Lindemann
Jan 15, 2009·The Journal of General Virology·Tatiana WiktorowiczAxel Rethwilm

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