Improved production of long-chain fatty acid in Escherichia coli by an engineering elongation cycle during fatty acid synthesis (FAS) through genetic manipulation

Journal of Microbiology and Biotechnology
Eunyoung JeonJinwon Lee

Abstract

The microbial biosynthesis of fatty acid of lipid metabolism, which can be used as precursors for the production of fuels of chemicals from renewable carbon sources, has attracted significant attention in recent years. The regulation of fatty acid biosynthesis pathways has been mainly studied in a model prokaryote, Escherichia coli. During the recent period, global regulation of fatty acid metabolic pathways has been demonstrated in another model prokaryote, Bacillus subtilis, as well as in Streptococcus pneumonia. The goal of this study was to increase the production of long-chain fatty acids by developing recombinant E. coli strains that were improved by an elongation cycle of fatty acid synthesis (FAS). The fabB, fabG, fabZ, and fabI genes, all homologous of E. coli, were induced to improve the enzymatic activities for the purpose of overexpressing components of the elongation cycle in the FAS pathway through metabolic engineering. The beta-oxoacyl-ACP synthase enzyme catalyzed the addition of acyl-ACP to malonyl-ACP to generate beta- oxoacyl-ACP. The enzyme encoded by the fabG gene converted beta-oxoacyl-ACP to beta-hydroxyacyl-ACP, the fabZ catalyzed the dehydration of beta-3-hydroxyacyl-ACP to trans-2-acyl-ACP, and the fa...Continue Reading

Citations

Jan 11, 2014·Biotechnology for Biofuels·Helge Jans Janßen, Alexander Steinbüchel
May 10, 2015·Applied Microbiology and Biotechnology·Changmin SungByung-Gee Kim
Mar 10, 2016·Journal of Biotechnology·Michael WierzbickiCong T Trinh
Jan 18, 2017·Journal of Industrial Microbiology & Biotechnology·Le MiXuetuan Wei
Mar 10, 2018·Protein Science : a Publication of the Protein Society·Carlos Rullán-LindAbel Baerga-Ortiz
Nov 2, 2014·Molecular BioSystems·Joris BeldMichael D Burkart
Feb 14, 2015·Chembiochem : a European Journal of Chemical Biology·Kara FinzelMichael D Burkart

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