Improved Production of Sublancin 168 Biosynthesized by Bacillus subtilis 168 Using Chemometric Methodology and Statistical Experimental Designs

BioMed Research International
Shengyue JiBinyun Cao

Abstract

Sublancin 168, as a distinct S-linked antimicrobial glycopeptide produced by Bacillus subtilis 168, is effective in killing specific microorganisms. However, the reported yield of sublancin 168 is at a low level of no more than 60 mg from 1 L fermentation culture of B. subtilis 168 by using the method in the literature. Thus optimization of fermentation condition for efficiently producing sublancin 168 is required. Here, Box-Behnken design was used to determine the optimal combination of three fermentation parameters, namely, corn powder, soybean meal, and temperature that were identified previously by Plackett-Burman design and the steepest ascent experiment. Subsequently, based on the response surface methodology, the quadratic regression model for optimally producing sublancin 168 was developed, and the optimal combination of culture parameters for maximum sublancin 168 production of 129.72 mg/L was determined as corn powder 28.49 g/L, soybean meal 22.99 g/L, and incubation temperature 30.8°C. The results showed that sublancin 168 production obtained experimentally was coincident with predicted value of 125.88 mg/L, and the developed model was proved to be adequate, and the aim of efficiently producing sublancin 168 was achi...Continue Reading

References

Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Aug 28, 2007·Journal of Bacteriology·Mark A StrauchYoann Le Breton
Jan 5, 2011·Nature Chemical Biology·Trent J OmanWilfred A van der Donk
Mar 5, 2013·Journal of Industrial Microbiology & Biotechnology·Ahmed Abd ElrazakJarka Glassey

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Software Mentioned

Minitab
Design Expert
SAS

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.