Improved propionic acid production from glycerol with metabolically engineered Propionibacterium jensenii by integrating fed-batch culture with a pH-shift control strategy

Bioresource Technology
Xin ZhugeJian Chen

Abstract

Propionic acid (PA) production with metabolically engineered Propionibacterium jensenii (pZGX04-gldA) was improved by integrating fed-batch culture with a two-stage pH control strategy in a 3-L fermenter. The following two-stage pH control strategy was used: the pH was controlled at 5.9 for 0-36 h and shifted to 6.5 after 36 h. The PA titer was increased to 21.43 g/L. On the basis of pH control, the influence of fed-batch culture on PA production was further investigated and the maximum PA production (34.62 g/L) was obtained when glycerol was fed at a constant rate of 3.33 mL/h from 60 to 132 h with an initial glycerol concentration of 25 g/L. Crude glycerol was then used to produce PA using the optimized strategies, and maximal PA production reached 37.26 g/L. The strategies may be useful for the production of PA by other propionibacteria species.

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Citations

Sep 27, 2014·Biotechnology and Bioengineering·Zhongqiang WangShang-Tian Yang
Nov 28, 2019·Applied Microbiology and Biotechnology·Ningzi Guan, Long Liu
Feb 6, 2017·Biotechnology Letters·Ismail EşMohamed Koubaa
Jan 8, 2021·Natural Product Reports·Yang LiXiulai Chen
Jul 25, 2021·Bioresource Technology·Yuexi ChenYinguang Chen
Aug 20, 2021·Applied Microbiology and Biotechnology·Ehab M Ammar, George P Philippidis

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