Improved protocol for high-quality co-extraction of DNA and RNA from rumen digesta

Folia Microbiologica
Milka PopovaDiego P Morgavi

Abstract

We report an improved method for total nucleic acids extraction from rumen content samples. The method employs bead beating, and phenol-chloroform extraction followed by saline-alcohol precipitation. Total nucleic acids and RNA yield and purity were assessed by spectrophotometric measurements; RNA integrity was estimated using Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer. The method provided total nucleic acids and RNA extracts of good quantity and quality. The extraction is not time consuming and it is valuable for ecological studies of rumen microbial community structure and gene expression.

References

Oct 12, 2012·World Journal of Microbiology & Biotechnology·Gabriela Villegas-RiveraJuan José Valdez-Alarcón
Dec 18, 2012·Applied and Environmental Microbiology·Milka PopovaC Martin
Oct 16, 2013·Anaerobe·Katerina FliegerovaR John Wallace
Jun 2, 2015·PloS One·Phillip R MyerHarvey C Freetly
Aug 13, 2015·PloS One·Sandrine Mignon-GrasteauIrène Gabriel
May 16, 2015·PloS One·Laura BaldoWalter Salzburger

Citations

Jan 1, 1994·Archives of Microbiology·R Wagner
Jun 9, 2005·Journal of Microbiological Methods·Saleema Saleh-LakhaJack T Trevors
Feb 2, 2006·BMC Molecular Biology·Andreas SchroederThomas Ragg
Jun 25, 2008·Journal of Microbiological Methods·Derek PersohGerhard Rambold

Related Concepts

Phenol
Rumen
Gene Expression
Genomics
Microbial
Phenols
Nucleic Acids
Microbiological Techniques
Metazoa
Chloroform

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