PMID: 6411304May 1, 1983Paper

Improved purification of beta-galactosidase from rabbit brain: two separable fractions share kinetic and structural properties

Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire
N L FraserJ W Callahan

Abstract

beta-Galactosidase has been purified from rabbit brain by a procedure which gives substantially greater purification and yield than the previously reported method. The enzyme was solubilized in 4% aqueous butanol and purified by a procedure which included affinity chromatography on columns of concanavalin A - Sepharose (ConA-Sepharose) and aminophenylthiogalactoside Sepharose (APT galactoside-Sepharose). The activity was resolved by DEAE-Sepharose chromatography into two fractions; the first did not bind to the column and was eluted in the unbound fraction, while the second bound to the column and could be eluted with a salt gradient. The unbound and bound fractions were purified 7600-fold and 4900-fold, respectively, by the newly developed procedure. Both gave two closely migrating protein bands on polyacrylamide gel electrophoresis and the major contaminating protein was beta-hexosaminidase in each case. The enzyme in the unbound fraction had an isoelectric point (pI) of 6.7 and an apparent molecular weight by gel filtration of 114 000 +/- 10 000. The enzyme that bound to DEAE-Sepharose at pH 8.0 and comprised about 60% of the total acid beta-galactosidase activity of rabbit brain eluted from Sephacryl S-200 as a single peak ...Continue Reading

Citations

Oct 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S HotamisligilS Raghavan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.