Improved purity and immunostimulatory activity of β-(1→3)(1→6)-glucan from Pleurotus sajor-caju using cell wall-degrading enzymes

Journal of Agricultural and Food Chemistry
Saranya SatitmanwiwatKhin Lay Kyu

Abstract

The objective of this work was to improve the purity of β-(1→3)(1→6)-glucan in the native triple helical structure from the fruiting bodies of Pleurotus sajor-caju for effective biological function using cell wall-degrading enzymes. A crude carbohydrate was extracted with hot water, then treated with crude xylanase and cellulase from Paenibacillus curdlanolyticus B-6. β-Glucan in the extract was purified to homogeneity with a single and symmetrical peak using 650M DEAE Toyopearl and Sepharose CL-6B column chromatography. The purity of β-glucan was confirmed by high-performance size-exclusion chromatography. Purified β-glucan was obtained at a purity of up to 90.2%. The Congo red reaction and atomic force microscopy indicated that the purified β-glucan exhibited a triple helix conformation. Purified β-glucan was able to effectively up-regulate the functions of macrophages such as nitric oxide (NO) and tumor necrosis factor (TNF-α) production.

References

Jan 30, 1999·Applied and Environmental Microbiology·K RatanakhanokchaiM Tanticharoen
Apr 25, 2000·Journal of Agricultural and Food Chemistry·M S IzydorczykB G Rossnagel
Nov 28, 2000·Carbohydrate Research·D ChenghuaX Huibi
Mar 24, 2006·Immunology Letters·M Y K LeungK P Fung
Apr 7, 2006·Applied and Environmental Microbiology·Patthra PasonKhanok Ratanakhanokchai
Sep 11, 2007·Mutation Research·Mário S MantovaniLúcia R Ribeiro
Jul 30, 2008·The Journal of Physical Chemistry. B·Xiaohua WangLina Zhang
Nov 18, 2009·Journal of Agricultural and Food Chemistry·Hsien-Yeh HsuLouis Kuoping Chao
Feb 5, 2010·Journal of Bioscience and Bioengineering·Somphit SornyothaKhanok Ratanakhanokchai

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Citations

Apr 3, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fei HuangZhencheng Wei
Jul 18, 2015·International Journal of Molecular Sciences·Jinsong WuJianquan Kan
Aug 9, 2015·Applied Microbiology and Biotechnology·Nicole DalonsoRegina Maria Miranda Gern
Aug 11, 2015·Carbohydrate Polymers·Sónia S FerreiraManuel A Coimbra
Aug 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qingye LiuLina Zhang
Apr 12, 2021·Carbohydrate Polymers·Xiaoyu GuoSteve W Cui
May 29, 2021·International Journal of Biological Macromolecules·Yuxi GuoPin Gong

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