PMID: 611363Jan 1, 1977Paper

Improved reversed passive hemagglutination for simple and rapid detection of staphycococcal enterotoxins A approximately E in food

Microbiology and Immunology
S YamadaT Terayama

Abstract

Detection and identification of staphylococcal enterotoxins in food or culture filtrates were performed using the reversed passive hemagglutination (RPHA) technique, with formalized sheep red blood cells (FSRBC) sensitized with immunologlobulins of anti-A, B, C, D, and E rabbit hyperimmune sera fractionated by affinity chromatography. The FSRBC sensitized with anti-A approximately E immunoglobulins showed a high level of reactivity and specificity in RPHA, against homologous types of purified enterotoxins and culture filtrates of toxin-producing strains. No non-specific reactions with various ingredients in foods nor cross-reactions among enterotoxin types were observed. The minimum amount of enterotoxins in foods detected by RPHA was calculated to be 0.01 micorgram/g without concentration, and the recovery rate of experimentally added toxins was calculated to be about 80%. Under routine laboratory practice, detection and identification of enterotoxins from incriminated foods of five food poisoning outbreaks were performed by RPHA within 3 hr after reception of the specimens. Among them, three were determined to be enterotoxin A food poisoning, one to be toxin C and the rest to be intoxication of A and D. The concentrations of ...Continue Reading

References

Dec 1, 1972·The Journal of Hygiene·R J GilbertM Simkovicová
Jan 1, 1974·Applied Microbiology·R ReiserM S Bergdoll
Nov 1, 1971·Infection and Immunity·M S BergdollK F Weiss
Jul 1, 1963·Journal of Bacteriology·E P CASMAN, R W BENNETT

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Citations

Dec 1, 1989·International Journal of Food Microbiology·C A Genigeorgis
Apr 1, 1994·International Journal of Food Microbiology·I G WilsonA Gilmour
Feb 22, 1986·Lancet·J DevenishJ Goold
Dec 1, 1984·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·S FukudaS Kimura
Jan 1, 1990·Society for Applied Bacteriology Symposium Series·H S Tranter, R D Brehm
Apr 1, 2000·Applied and Environmental Microbiology·N K SharmaC E Dodd
Feb 1, 1982·Japanese Journal of Medical Science & Biology·K ShinagawaG Sakaguchi
Mar 1, 1986·Journal of Clinical Microbiology·H IgarashiM S Bergdoll

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