Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability

Journal of Applied Microbiology
N GurdoM A Galvagno

Abstract

To investigate multiple tolerance of Saccharomyces cerevisiae obtained through a laboratory strategy of adaptive evolution in acetic acid, its relation with enzymatic ROS detoxification and bioethanol 2G production. After adaptive evolution in acetic acid, a clone (Y8A) was selected for its tolerance to high acetic acid concentrations (13 g l-1 ) in batch cultures. Y8A was resistant to multiple stresses: osmotic, thermic, oxidative, saline, ethanol, organic acid, phenolic compounds and slow freeze-thawing cycles. Also, Y8A was able to maintain redox homeostasis under oxidative stress, whereas the isogenic parental strain (Y8) could not, indicating higher basal activity levels of antioxidative enzyme Catalase (CAT) and Gluthatione S-transferase (GST) in Y8A. Y8A reached higher bioethanol levels in a fermentation medium containing up to 8 g l-1 of acetic acid when compared to parental strain Y8. A multiple-stress-tolerant clone was obtained using adaptive evolution in acetic acid. Stress cross-tolerance could be explained by its enzymatic antioxidative capacity, namely CAT and GST. We demonstrate that adaptive evolution used in S. cerevisiae was a useful strategy to obtain a yeast clone tolerant to multiple stresses. At the same ...Continue Reading

References

Apr 1, 1974·The Biochemical Journal·R C BrayL Calabrese
Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Jan 1, 1984·Methods in Enzymology·H Aebi
Feb 1, 1996·Molecular Microbiology·P Moradas-FerreiraW Mager
Jan 1, 1997·Journal of Industrial Microbiology & Biotechnology·J G LewisK Watson
Jan 6, 2001·Redox Report : Communications in Free Radical Research·P Moradas-Ferreira, V Costa
Jul 16, 2003·Biochemical and Biophysical Research Communications·Lei ZhangAkinori Ohta
Mar 23, 2005·FEMS Yeast Research·Z Petek CakarUwe Sauer
Apr 7, 2006·Applied and Environmental Microbiology·Pablo I NikelBeatriz S Méndez
Oct 12, 2007·Journal of Applied Microbiology·M F KronbergM A Galvagno
Feb 27, 2008·Biochimica Et Biophysica Acta·Gabriel G PerroneIan W Dawes
Oct 6, 2009·Applied and Environmental Microbiology·Ken UkibeHiroshi Takagi
Oct 22, 2013·Biotechnology for Biofuels·Valeria Wallace-Salinas, Marie F Gorwa-Grauslund
Jul 5, 2015·Applied Microbiology and Biotechnology·Janja TrčekLaura R Jarboe
Nov 26, 2015·Applied Microbiology and Biotechnology·Seung-Ho BaekJi-Sook Hahn

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