Improved Salt Tolerance and Metabolomics Analysis of Synechococcus elongatus UTEX 2973 by Overexpressing Mrp Antiporters

Frontiers in Bioengineering and Biotechnology
Jinyu CuiWeiwen Zhang

Abstract

The fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 (Syn2973) is a promising candidate for photosynthetic microbial factory. Seawater utilization is necessary for large-scale cultivation of Syn2973 in the future. However, Syn2973 is sensitive to salt stress, making it necessary to improve its salt tolerance. In this study, 21 exogenous putative transporters were individually overexpressed in Syn2973 to evaluate their effects on salt tolerance. The results showed the overexpression of three Mrp antiporters significantly improved the salt tolerance of Syn2973. Notably, overexpressing the Mrp antiporter from Synechococcus sp. PCC 7002 improved cell growth by 57.7% under 0.4 M NaCl condition. In addition, the metabolomics and biomass composition analyses revealed the possible mechanisms against salt stress in both Syn2973 and the genetically engineered strain. The study provides important engineering strategies to improve salt tolerance of Syn2973 and is valuable for understanding mechanisms of salt tolerance in cyanobacteria.

References

Jan 1, 1977·Annual Review of Microbiology·R Y Stanier, G Cohen-Bazire
Nov 1, 1988·Journal of General Microbiology·J E HoldsworthC Ratledge
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Rungaroon WaditeeTeruhiro Takabe
Mar 16, 2005·Analytical Biochemistry·Tatsuya MasukoYuan C Lee
Jun 28, 2005·Extremophiles : Life Under Extreme Conditions·Talia H SwartzTerry Ann Krulwich
Aug 18, 2009·Molecular Microbiology·Terry A KrulwichMasahiro Ito
Aug 25, 2009·Applied and Environmental Microbiology·Fuminori FukayaTeruhiro Takabe
May 9, 2015·Photosynthesis Research·Hiroko TaharaHisataka Ohta
May 31, 2015·Trends in Microbiology·Aindrila Mukhopadhyay
Jun 14, 2015·Microbiological Research·Sumaya Al-HosaniLina F Yousef
Feb 18, 2016·Applied Microbiology and Biotechnology·Xinyan GaoWeiwen Zhang
Apr 16, 2016·Applied Microbiology and Biotechnology·Kuo SongXuefeng Lu
Feb 1, 2017·Scientific Reports·Thomas J MuellerCostas D Maranas
Dec 9, 2017·Frontiers in Microbiology·Masahiro ItoTerry A Krulwich
May 15, 2018·Cell·Hongjun YuHuilin Li
Aug 22, 2018·Biotechnology for Biofuels·Xiaoming TanWolfgang R Hess
Nov 10, 2018·Proceedings of the National Academy of Sciences of the United States of America·Justin UngererHimadri B Pakrasi
Apr 13, 2019·Biotechnology Advances·Guodong LuanXuefeng Lu
Jul 10, 2019·ACS Synthetic Biology·Cory J KnootHimadri B Pakrasi

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Methods Mentioned

BETA
environmental stresses
fatty

Software Mentioned

MEGA
ClustalW
Qualitative Analysis
Xcalibur

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