Improved sectioning in a slit scanning confocal microscope

Optics Letters
Vincent PoherMark A A Neil

Abstract

We describe a simple implementation of a slit scanning confocal microscope to obtain an axial resolution better than that of a point-scanning confocal microscope. Under slit illumination, images of a fluorescent object are captured using an array detector instead of a line detector so that out-of-focus light is recorded and then subtracted from the adjacent images. Axial resolution after background subtraction is 2.2 times better than the slit confocal resolution, and out-of-focus image suppression is calculated to attenuate with defocus faster by 1 order of magnitude than in the point confocal case.

References

Jun 30, 2006·Applied Optics·Rainer Heintzmann, Pier A Benedetti

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Citations

Feb 10, 2018·Methods and Applications in Fluorescence·J VangindertaelK P F Janssen
Nov 13, 2017·Journal of Biomedical Optics·Yubo TangRebecca R Richards-Kortum
Jul 5, 2016·Biomedical Optics Express·Michael Hughes, Guang-Zhong Yang

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