Improved sensitivity and decreased sample size in a cytotoxicity test for biomaterials: a modified colony microassay using a microplate and crystal violet staining

Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials
T TsuchiyaA Nakamura

Abstract

Modified Eagle's minimum essential medium supplemented with 5% fetal calf serum was highly sensitive to cytotoxicity and formed large control colonies in the V79 colony assay. A highly sensitive cytotoxicity test was developed using 96-well microtiter plates. Test chemicals or extracts of polyurethane materials containing the same chemicals were added 24 h after inoculation of cell suspensions. The cells were fixed and stained with crystal violet after additional culture for 6 days (V79 cells) or 10 days (Balb/3T3 cells). In terms of sensitivity and rapid quantitative measurement, this modified colony microassay, using a low cell density in 96-well microplates, was superior to various cytotoxicity tests such as colony, growth inhibition, cytolethality, and agar diffusion assays.

References

Jul 1, 1983·Journal of Biomedical Materials Research·H J JohnsonR F Wallin
Jul 1, 1993·Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials·T TsuchiyaA Nakamura
Jan 1, 1989·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K SaotomeM Umeda

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Citations

Aug 30, 2005·Yonsei Medical Journal·Hyun Sook BaekJong-Chul Park
Dec 16, 2005·Journal of Biomedical Materials Research. Part a·Nasreen BanuRumi Sawada

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