Improved sensitivity and specificity for prostate cancer diagnosis based on the urine PCA3/PSA ratio acquired by sequence‑specific RNA capture.

Oncology Reports
Kewen ZhengLingjun Kong

Abstract

Prostate cancer antigen 3 (PCA3) is a non-coding RNA fragment that is overexpressed in prostate cancer cells. However, the clinical applications of PCA3 are highly limited due to the instability of RNA and the lack of reliable and efficient RNA extraction and purification methods. Thus, in the present study, we compared three different methods of RNA extraction to further confirm the higher yield of commercial magnetic beads with poly-T functionalization and a capturer strand. The current protocols for RNA extraction of i) the phenol-chloroform method, ii) the affinity column method and iii) magnetic beads with poly-T functionalization and a capturer strand were applied separately for RNA extraction in urine samples. Reverse transcription‑quantitative polymerase chain reaction was performed to evaluate the yield of the three methods of RNA extraction. Furthermore, 52 urine samples after prostate massage from patients suspected of a diagnosis of prostate cancer were collected. The Mag-Cap method and RT-PCR were applied to obtain the PCA3 score. The clinical value of the PCA3 score was investigated by comparison with the pathology of the prostate biopsy. The yield of the Mag-Cap method was higher than that of the phenol‑chlorofor...Continue Reading

References

Jul 1, 1992·Cancer·J D Schmidt
May 1, 1995·CA: a Cancer Journal for Clinicians·K M SlawinP T Scardino
Jun 20, 2003·European Urology·Daphne HesselsJack A Schalken
Nov 11, 2003·Urology·Hans Lilja
May 28, 2004·The New England Journal of Medicine·Ian M ThompsonCharles A Coltman
Mar 30, 2007·CA: a Cancer Journal for Clinicians·Robert A SmithHarmon J Eyre
Jan 13, 2010·Nature Reviews. Urology·Guillaume Ploussard, Alexandre de la Taille
Nov 26, 2010·Cancer Letters·John R DayHarry Rittenhouse
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Feb 9, 2011·Current Opinion in Urology·Monique J Roobol
May 27, 2011·Acta Oncologica·Monique J RoobolAnders Bjartell
Jun 16, 2012·CA: a Cancer Journal for Clinicians·Rebecca SiegelElizabeth Ward
Mar 5, 2013·Chinese Journal of Cancer·Wanqing ChenJie He
Mar 20, 2013·BioMed Research International·Valentina CasadioWainer Zoli
Oct 12, 2013·CA: a Cancer Journal for Clinicians·Avnesh S Thakor, Sanjiv S Gambhir
Jan 31, 2014·Frontiers in Physiology·Joshua McCarrollPhoebe A Phillips
Feb 21, 2014·Science Translational Medicine·Chetan BettegowdaLuis A Diaz
Apr 16, 2014·Journal of Nanoscience and Nanotechnology·Bruno Campos JanegitzValtencir Zucolotto

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Citations

Nov 26, 2019·Oncotarget·Ana Emília Goulart LemosEtel Rodrigues Pereira Gimba
Oct 1, 2020·Sensors·Clara Abardía-SerranoMaría Jesús Lobo-Castañón
Jul 17, 2020·Cancer Management and Research·Christopher J McNallyDeclan J McKenna
Jul 3, 2021·International Journal of Molecular Sciences·Riccardo Di FioreJean Calleja-Agius

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