PMID: 12772701May 30, 2003Paper

Improved specificity of Y-STR typing in DNA mixture samples

International Journal of Legal Medicine
Walther ParsonBurkhard Berger

Abstract

Y-STR loci are beneficial for the analysis of forensic samples especially in sexual assault cases or other situations where mixtures of male and female cells are present. However, the amplification of Y-chromosomal STRs is also known to result in the formation of artefactual amplification products, mainly due to insufficient PCR specificity. This is a major drawback of the method, as the sensitivity as well as the correct Y-STR interpretation are affected. In our study, the addition of a PCR enhancer to the reaction master-mix resulted in a significant increase of specificity of Y-STR typing. This was clearly demonstrated by a loss of artefactual signal with increasing enhancer concentration, while the peak heights of the Y-STR alleles were not significantly affected by the enhancer. Mixtures of up to 1:500 (200 pg male and 100 ng female DNA) gave correct Y-STR profiles when the PCR enhancer was added to the reaction, while artefactual amplification succeeded over Y-specific amplification when no PCR enhancer was present.

Citations

Nov 27, 2004·International Journal of Legal Medicine·Frederick C DelfinMaria Corazon A De Ungria
Mar 28, 2008·Journal of Forensic Sciences·Kathleen A Mayntz-PressJack Ballantyne
Nov 11, 2016·International Journal of Legal Medicine·Wenqiong ZhangDaixin Huang
Mar 29, 2006·Journal of Forensic Sciences·Chien-Wei ChangLori K Hennessy
Oct 19, 2005·International Journal of Legal Medicine·Ulrike SchmidtWolfgang Mann
Feb 8, 2005·International Journal of Legal Medicine·K AnslingerW Eisenmenger
Nov 10, 2006·International Journal of Legal Medicine·Sabine Lutz-BonengelUlrike Schmidt
May 8, 2018·International Journal of Legal Medicine·HaiJun HeJian Huang
Feb 20, 2021·Legal Medicine·Tomomi OtagiriHideki Asamura
Jun 15, 2005·Analytical Chemistry·T A BrettellR Saferstein

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