PMID: 6171055Jul 1, 1981Paper

Improved stability of methanolic wright's stain with additive reagents

Stain Technology
J C LiaoC Patel

Abstract

Additive reagents have been investigated to improve the stability of methanolic Wright's stain. The addition of ammonium halides, monoalkylamine hydrochlorides, dialkylamine hydrochlorides or trialkylamine hydrochlorides to methanolic Wright's stain was found to enhance the stability of stain components in methanol. No change in performance is observed with these additives present. Random precipitation in the stain solution was still observed with the addition of ammonium halides and monoalkylamine hydrochlorides. No precipitation was found in stain solutions containing hydrochlorides of most dialkylamines and trialkylamines. Of the compounds evaluated, 0.6% diethylamine hydrochloride added to methanolic stain solutions produced the most desirable overall results. Mechanisms of stabilization and precipitation in these stain solutions are proposed. Essentially, separation of the thiazine-eosinate ion pair through interaction with an appropriate additive increases stain stability. The solubilities of thiazine-eosinate or additive cation-eosinate ion pairs in methanol determine the formation of precipitate in such stain solutions.

References

Sep 15, 1976·Journal of Chromatography·W W DeanM Stastny
Jan 1, 1977·Stain Technology·W W DeanG J Lubrano
Jan 1, 1978·The Histochemical Journal·P N Marshall
Nov 1, 1975·Journal of Clinical Pathology·P N MarshallS M Lewis
May 1, 1979·Stain Technology·J W GillilandG J Lubrano

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Citations

May 15, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·H J Nelis, A P De Leenheer
Jan 1, 1985·British Journal of Haematology·M BinsM R Halie

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