Improved Stable Isotope Dilution Assay for Dietary Folates Using LC-MS/MS and Its Application to Strawberries

Frontiers in Chemistry
Lisa StriegelMichael Rychlik

Abstract

Folates play an important role in the human body and a deficiency of this vitamin can cause several diseases. Therefore, a reliable analytical method is crucial for the determination of folate vitamers in strawberries and other dietary folate sources. A stable isotope dilution LC-MS/MS method for analyzing folates in food was developed and validated. The folate vitamers Pteroylmonoglutamic acid, tetrahydrofolate, 5-methyltetrahydrofolate, and 5-formyltetrahydrofolate were quantified using13C-labeled internal standards. Validation of the assay was accomplished by determining linearity, precision, recovery, limit of detection, and limit of quantification and revealed to be a precise, sensitive, and accurate method to determine folate vitamers. Strawberries are worldwide consumed and known to be a good dietary source of nutritive compounds. Using this method, folate concentrations in selected commercial strawberry cultivars and experimental breeding lines grown in Germany and Australia were investigated. Total folates varied from 59 to 153 μg/100 g on fresh weight basis. Furthermore, folate content after lyophilizing or freezing did not show any significant differences compared to fresh strawberries. However, significant losses of...Continue Reading

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Citations

Aug 29, 2019·Foods·Lisa StriegelMichael Rychlik
Oct 11, 2019·Current Developments in Nutrition·Jette JakobsenMichael Rychlik
Dec 14, 2018·Frontiers in Nutrition·Lisa StriegelMichael Rychlik
Feb 11, 2020·Frontiers in Bioengineering and Biotechnology·Dirk Volker WoortmanMichael Rychlik
Sep 12, 2020·Food Chemistry·Fanny CoffigniezAurélien Briffaz

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