Improved sugar-free succinate production by Synechocystis sp. PCC 6803 following identification of the limiting steps in glycogen catabolism

Metabolic Engineering Communications
Tomohisa HasunumaAkihiko Kondo

Abstract

Succinate produced by microorganisms can replace currently used petroleum-based succinate but typically requires mono- or poly-saccharides as a feedstock. The cyanobacterium Synechocystis sp. PCC6803 can produce organic acids such as succinate from CO2 not supplemented with sugars under dark anoxic conditions using an unknown metabolic pathway. The TCA cycle in cyanobacteria branches into oxidative and reductive routes. Time-course analyses of the metabolome, transcriptome and metabolic turnover described here revealed dynamic changes in the metabolism of Synechocystis sp. PCC6803 cultivated under dark anoxic conditions, allowing identification of the carbon flow and rate-limiting steps in glycogen catabolism. Glycogen biosynthesized from CO2 assimilated during periods of light exposure is catabolized to succinate via glycolysis, the anaplerotic pathway, and the reductive TCA cycle under dark anoxic conditions. Expression of the phosphoenolpyruvate (PEP) carboxylase gene (ppc) was identified as a rate-limiting step in succinate biosynthesis and this rate limitation was alleviated by ppc overexpression, resulting in improved succinate excretion. The sugar-free succinate production was further enhanced by the addition of bicarbon...Continue Reading

Citations

Apr 30, 2019·Biotechnology and Bioengineering·Romina Lasry TestaSoledad M Diaz
Apr 17, 2019·Scientific Reports·Shoki ItoTakashi Osanai
Nov 17, 2017·Biotechnology and Bioengineering·Christian DavidKatja Bühler
Jan 10, 2017·Frontiers in Microbiology·Yuko TomitaTakashi Osanai

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Methods Mentioned

BETA
genetic modifications
PCR
chip

Software Mentioned

MassHunter Workstation Data Acquisition

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