PMID: 15329481Aug 27, 2004Paper

Improved survival of vitrified in vivo-derived porcine embryos

The Journal of Reproduction and Development
Hitoshi UshijimaHiroshi Nagashima

Abstract

An efficient cryopreservation protocol for porcine morulae was investigated with three types of vitrification having different cooling rates (Exp. 1). Survival of embryos vitrified after removal of cytoplasmic lipid droplets was also examined by means of the minimum volume cooling (MVC) method (Exp. 2). In Exp. 1, the morula stage embryos were vitrified with a 0.25 ml plastic straw (ST-method), gel loading tip (GLT-method) and the MVC-method, respectively, and stored in liquid nitrogen after which they were warmed in sucrose solutions with cryoprotectants being subsequently removed in a stepwise manner. In Exp. 2, morulae were centrifuged with 7.5 microg/ml cytocharasin B at 12000 x g for 20 min to polarize the cytoplasmic lipid droplets that were then removed from the embryos by micromanipulation (delipation). Both those delipated at the morula stage and the intact embryos at the morula to blastocyst stages were vitrified by the MVC-method. In vitro survival of the vitrified embryos was assessed in both experiments by culturing in NCSU-23 + 10% FCS for 48 h. In vitro developments of vitrified embryos after warming to blastocysts were 20% (6/30) for the ST-method, 39% (18/46) for the GLT-method, and 60% (26/43) for the MVC-meth...Continue Reading

References

Mar 30, 1995·Nature·H NagashimaM B Nottle
Oct 1, 1994·Biology of Reproduction·H NagashimaM B Nottle
Nov 14, 1997·The Veterinary Record·M KuwayamaH Callesen
Dec 25, 2002·Theriogenology·C GalliG Lazzari
Apr 1, 1992·Theriogenology·H NagashimaH Niemann

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Citations

Mar 8, 2005·The Journal of Reproduction and Development·Hitoshi Ushijima
Dec 26, 2009·The Journal of Reproduction and Development·Nobutada SakagamiKoji Yoshioka
Jan 19, 2011·The Journal of Reproduction and Development·Kazuaki NakanoHiroshi Nagashima
Jun 30, 2015·The Journal of Reproduction and Development·Kanchana PunyawaiRangsun Parnpai
Jan 4, 2013·Reproduction in Domestic Animals = Zuchthygiene·H Suzuki
Feb 6, 2016·PloS One·Francisco Marco-JiménezJosé Salvador Vicente
Aug 12, 2017·Animal Science Journal = Nihon Chikusan Gakkaihō·Yoshiyuki Nakamura, Kazuhiro Kikuchi
Apr 5, 2016·The Journal of Veterinary Medical Science·Tatsuya HoriToshihiko Tsutsui
Jul 13, 2012·The Journal of Reproduction and Development·Hitomi MatsunariHiroshi Nagashima
Feb 2, 2010·Reproductive Medicine and Biology·Yasuyuki AbeHiroshi Suzuki
Sep 7, 2012·Reproduction, Fertility, and Development·Gábor Vajta
Feb 25, 2021·Biopreservation and Biobanking·Du XingzhuFu Xiangwei

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