Improved technique for immunoelectron microscopy. How to prepare epoxy resin to obtain approximately the same immunogold labeling for epoxy sections as for acrylic sections without any etching

Micron : the International Research and Review Journal for Microscopy
S-H Brorson, F Skjørten

Abstract

The purpose of this study was to improve the immunogold labeling of epoxy sections and to increase our knowledge of the mechanism for how antigens become immunolabeled on resin sections. Tissues from pancreas, thyroid and fibrin clots were embedded in an epoxy resin and LR-White. The epoxy mixture was composed and treated in different ways, especially with respect to altered amounts of accelerator (DMP-30). Immunogold labeling was performed with anti-glucagon, anti-thyroglobulin and anti-fibrinogen respectively. By increasing the amount of DMP-30 in the infiltration steps and/or embedding step, we observed a significant rise in the immunogold labeling. For the largest proteins the labeling was up to 8 times more intense than the labeling achieve with epoxy sections produced by 'normal' amount of accelerator in the embedding mixture and without accelerator in the infiltration mixture. For the smallest protein, glucagon, the differences were almost absent. The labeling of thyroglobulin and fibrinogen on the high accelerator epoxy sections was up to 70% of the labeling of LR-White sections, while conventional epoxy sections showed a labeling of 5-10% of that obtained with acrylic labeling. The cutting qualities of the high-acceler...Continue Reading

References

May 1, 1988·Ultrastructural Pathology·R HolmJ V Johannessen
Sep 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E KellenbergerM Wurtz
Sep 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G R Newman, J A Hobot
Jan 1, 1994·Micron : the International Research and Review Journal for Microscopy·S H BrorsonF Skjørten
Dec 1, 1993·Acta histochemica·M Kasper, A Migheli
Jan 1, 1995·Micron : the International Research and Review Journal for Microscopy·S H Brorson, F Skjørten
Jun 1, 1996·Micron : the International Research and Review Journal for Microscopy·S H Brorson, F Skjørten

❮ Previous
Next ❯

Citations

Aug 11, 2000·Micron : the International Research and Review Journal for Microscopy·S H Brorson
Feb 13, 2001·Micron : the International Research and Review Journal for Microscopy·S H Brorson, G H Nguyen
Oct 1, 1996·Micron : the International Research and Review Journal for Microscopy·S H Brorson
Jun 1, 1997·Micron : the International Research and Review Journal for Microscopy·S H Brorson
Aug 4, 1999·Micron : the International Research and Review Journal for Microscopy·S H Brorson
Nov 2, 1999·Micron : the International Research and Review Journal for Microscopy·S H BrorsonS Rashid
Aug 11, 2000·Micron : the International Research and Review Journal for Microscopy·S H BrorsonI K Woxen
Feb 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·S H BrorsonF Skjørten
Dec 10, 2008·Journal of Immunological Methods·Fabio D'AmicoFranca Stivala
Mar 11, 2000·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·S H Brorson
May 23, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Shuji Yamashita, Yasunori Okada
Jan 2, 2007·Progress in Histochemistry and Cytochemistry·Shuji Yamashita

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.