Improved TNT detoxification by starch addition in a nitrogen-fixing Methylophilus-dominant aerobic microbial consortium

Journal of Hazardous Materials
Muhammad Imran KhanJoonhong Park

Abstract

In this study, a novel aerobic microbial consortium for the complete detoxification of 2,4,6-trinitrotoluene (TNT) was developed using starch as a slow-releasing carbon source under nitrogen-fixing conditions. Aerobic TNT biodegradation coupled with microbial growth was effectively stimulated by the co-addition of starch and TNT under nitrogen-fixing conditions. The addition of starch with TNT led to TNT mineralization via ring cleavage without accumulation of any toxic by-products, indicating improved TNT detoxification by the co-addition of starch and TNT. Pyrosequencing targeting the bacterial 16S rRNA gene suggested that Methylophilus and Pseudoxanthomonas population were significantly stimulated by the co-addition of starch and TNT and that the Methylophilus population became predominant in the consortium. Together with our previous study regarding starch-stimulated RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) degradation (Khan et al., J. Hazard. Mater. 287 (2015) 243-251), this work suggests that the co-addition of starch with a target explosive is an effective way to stimulate aerobic explosive degradation under nitrogen-fixing conditions for enhancing explosive detoxification.

References

Jan 1, 1997·Archives of Environmental Contamination and Toxicology·R BoopathyC F Kulpa
May 1, 1997·Canadian Journal of Microbiology·J L MartinR A Drijber
May 2, 2000·Applied and Environmental Microbiology·S F NishinoJ C Spain
Oct 31, 2000·Applied and Environmental Microbiology·J W PakG H Chambliss
Aug 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·A Esteve-NúñezJ L Ramos
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jun 9, 2004·Applied and Environmental Microbiology·Richard E WilliamsNeil C Bruce
Jul 29, 2005·Environmental Science & Technology·Jacqueline M Tront, Joseph B Hughes
Apr 7, 2006·Applied and Environmental Microbiology·Kirsti M RitalahtiFrank E Löffler
May 31, 2007·Applied Microbiology and Biotechnology·Barth F SmetsAbraham Esteve-Nuñez
Apr 18, 2008·Briefings in Bioinformatics·Sudhir KumarKoichiro Tamura
May 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yongliang YanQi Jin
Nov 14, 2008·Nucleic Acids Research·J R ColeJ M Tiedje
Jun 23, 2009·Current Microbiology·Bheong-Uk LeeKye-Heon Oh
Jul 9, 2009·FEMS Microbiology Letters·Steffen Kolb
Jul 25, 2009·International Journal of Systematic and Evolutionary Microbiology·Munusamy MadhaiyanTong-Min Sa
Mar 30, 2010·Journal of Hazardous Materials·Kaidar AyoubAlain Bermond
Mar 8, 2011·International Journal of Systematic and Evolutionary Microbiology·Nina V DoroninaYuri A Trotsenko
Jan 17, 2012·Journal of Environmental Management·Olga MuterVadims Bartkevics
Jun 26, 2012·Journal of Environmental Management·Mason ErkelensAndrew S Ball
Aug 29, 2012·Biotechnology and Bioengineering·Julie C ChambonPhilip J Binning
Nov 21, 2012·Journal of Hazardous Materials·Krishna M LamichhaneSusan Schenck
Feb 19, 2014·Archives of Environmental Contamination and Toxicology·Sumit K SoniAlok Kalra
Feb 20, 2014·Environmental Toxicology and Chemistry·Chih-Ching ChienChien-Cheng Chen

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