Improved understanding of biofilm development by Piscirickettsia salmonis reveals potential risks for the persistence and dissemination of piscirickettsiosis.

Scientific Reports
Héctor A LevipanRuben Avendaño-Herrera

Abstract

Piscirickettsia salmonis is the causative agent of piscirickettsiosis, a disease with high socio-economic impacts for Chilean salmonid aquaculture. The identification of major environmental reservoirs for P. salmonis has long been ignored. Most microbial life occurs in biofilms, with possible implications in disease outbreaks as pathogen seed banks. Herein, we report on an in vitro analysis of biofilm formation by P. salmonis Psal-103 (LF-89-like genotype) and Psal-104 (EM-90-like genotype), the aim of which was to gain new insights into the ecological role of biofilms using multiple approaches. The cytotoxic response of the salmon head kidney cell line to P. salmonis showed interisolate differences, depending on the source of the bacterial inoculum (biofilm or planktonic). Biofilm formation showed a variable-length lag-phase, which was associated with wider fluctuations in biofilm viability. Interisolate differences in the lag phase emerged regardless of the nutritional content of the medium, but both isolates formed mature biofilms from 288 h onwards. Psal-103 biofilms were sensitive to Atlantic salmon skin mucus during early formation, whereas Psal-104 biofilms were more tolerant. The ability of P. salmonis to form viable an...Continue Reading

References

Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Apr 1, 1997·Emerging Infectious Diseases·J L Fryer, M J Mauel
Aug 4, 1998·Applied and Environmental Microbiology·S MarshallC Orrego
Nov 5, 1999·Diseases of Aquatic Organisms·P A SmithJ Larenas
Sep 8, 2000·Microbial Ecology·A R RiceA K Camper
Jun 5, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·M D FastN W Ross
Jun 19, 2002·Veterinary Microbiology·Michael J Mauel, Debra L Miller
Sep 3, 2003·FEMS Microbiology Letters·Andrés CasanovaMatilde Jashés M
Sep 10, 2003·Journal of Fish Diseases·J L Fryer, R P Hedrick
Jul 10, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Sangeetha SubramanianNeil W Ross
Mar 6, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Michael J MauelPedro A Smith
Mar 3, 2009·Applied and Environmental Microbiology·Sébastien VilainVolker S Brözel
Feb 19, 2010·Applied Microbiology and Biotechnology·Paolo LandiniReindert Nijland
Jul 20, 2010·Applied and Environmental Microbiology·Otini KroukampGideon M Wolfaardt
Mar 17, 2012·Diseases of Aquatic Organisms·A J YañezJ G Carcamo
Jun 12, 2013·Environmental Science & Technology·Erik R ZettlerLinda A Amaral-Zettler
Jun 27, 2013·International Journal of Food Microbiology·Sachin R KadamTjakko Abee
Oct 9, 2013·Fish & Shellfish Immunology·Daniela GomezIrene Salinas
Nov 28, 2013·Journal of Fish Diseases·M Rozas, R Enríquez
Dec 7, 2013·Critical Reviews in Microbiology·Mira Okshevsky, Rikke Louise Meyer
Jan 17, 2014·Diseases of Aquatic Organisms·R Avendaño-HerreraA E Toranzo
Apr 9, 2014·Veterinary Microbiology·Said BenhamedMaría Ángeles Esteban
Nov 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Albert SiryapornZemer Gitai
Apr 15, 2016·Nature Communications·Lynne TurnbullCynthia B Whitchurch
May 14, 2016·Molecular BioSystems·Monica Fengsrud Brinchmann
Aug 12, 2016·Nature Reviews. Microbiology·Hans-Curt FlemmingStaffan Kjelleberg
Oct 4, 2016·Expert Review of Vaccines·Kevin MaiseyMyron Christodoulides
Nov 22, 2016·Critical Reviews in Microbiology·Joana AzeredoClaus Sternberg
Nov 29, 2016·Microbial Pathogenesis·A I IsiakuM B Bello
Sep 26, 2017·Nature Reviews. Microbiology·Hyun KooLuanne Hall-Stoodley
Oct 17, 2017·Frontiers in Cellular and Infection Microbiology·Cristian OliverAlejandro J Yáñez

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
scraping
Protein Assay

Software Mentioned

betareg
R package
emmeans R package
R
emmeans ’ R package
propagate

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.