Improved vectors and genome-wide libraries for CRISPR screening

BioRxiv : the Preprint Server for Biology
Neville E SanjanaFeng Zhang

Abstract

Genome-wide, targeted loss-of-function pooled screens using the CRISPR (clustered regularly interspaced short palindrome repeats)–associated nuclease Cas9 in human and mouse cells provide an alternative screening system to RNA interference (RNAi) and have been used to reveal new mechanisms in diverse biological models[1][1]–[4][2]. Previously, we used a Genome-scale CRISPR Knock-Out (GeCKO) library to identify loss-of-function mutations conferring vemurafenib resistance in a melanoma model[1][1]. However, initial lentiviral delivery systems for CRISPR screening had low viral titer or required a cell line already expressing Cas9, limiting the range of biological systems amenable to screening. [1]: #ref-1 [2]: #ref-4

Related Concepts

Birth
Genetic Vectors
Genome
Melanoma
Cell Line, Tumor
Gene Mutation
RNA Interference
Protein Expression
Mouse Cell Line
Resistance Process

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