Improved viability of Lactobacillus acidophilus NRRL-B 4495 during freeze-drying in whey protein-pullulan microcapsules

Journal of Microencapsulation
Burcu Çabuk, Şebnem Tellioglu Harsa

Abstract

In this research, pullulan was incorporated in protein-based encapsulation matrix in order to assess its cryoprotective effect on the viability of freeze-dried (FD) probiotic Lactobacillus acidophilus NRRL-B 4495. This study demonstrated that pullulan in encapsulation matrix resulted in a 90.4% survival rate as compared to 88.1% for whey protein (WPI) encapsulated cells. The protective effects of pullulan on the survival of FD-encapsulated cells in gastrointestinal conditions were compared. FD WPI-pullulan capsules retained higher survived cell numbers (7.10 log CFU/g) than those of FD WPI capsules (6.03 log CFU/g) after simulated gastric juice exposure. Additionally, use of pullulan resulted in an increased viability after bile exposure. FD-free bacteria exhibited 2.18 log CFU/g reduction, while FD WPI and FD WPI-pullulan encapsulated bacteria showed 0.95 and 0.49 log CFU/g reduction after 24 h exposure to bile solution, respectively. Morphology of the FD microcapsules was visualized by scanning electron microscopy.

References

Oct 1, 1995·Applied and Environmental Microbiology·S B LeslieL M Crowe
Mar 10, 2001·Journal of Dairy Science·M E Sanders, T R Klaenhammer
Apr 4, 2003·The Journal of Nutrition·Bryan W WolfKevin C Maki
Dec 9, 2008·International Journal of Food Microbiology·Gildas Komenan GbassiEric Marchioni
May 30, 2009·Environmental Health Perspectives·Melissa Lee Phillips
Apr 28, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Beatrice AlbertiniPatrizia Brigidi
Jul 31, 2012·Applied and Environmental Microbiology·Dianawati DianawatiNagendra P Shah
Aug 31, 2013·Carbohydrate Polymers·Walfred Rosas-FloresJuan Alfredo Salazar-Montoya

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