DOI: 10.1101/488585Dec 10, 2018Paper

Improved yield of AAV2 and rAAV2-retro serotypes following sugar supplementation during the viral production phase

BioRxiv : the Preprint Server for Biology
Meghan RegoMelina Fan

Abstract

Hyperosmolarity has been used as a means to improve yields in various processes such as antibody production in hybridoma cells and retroviral and adenoviral production systems. In this study we demonstrate that treatment of AAVpro 293T packaging cells with the sugars sucrose or sorbitol during adeno-associated virus (AAV) production improves the yields of AAV2 and rAAV2-retro serotypes 1.8-fold and 1.5-fold, respectively. Using an iodixanol gradient centrifugation strategy followed by a column-based buffer exchange, we show that the sugar supplements are not carried over to the final viral preparation and have no effect on the purity or infectivity of the virus. Given the wide availability, low cost, and ease of use of these sugars, we believe that this strategy can be easily adopted by vector cores and laboratories to improve yields of their AAV preparations.

Related Concepts

Dependovirus
Buffers
Centrifugation
Disease Vectors
Genes, env
Hybridomas
Laboratory
Sorbitol
Virus Replication
Virus

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