Improvement and optimization of a multiplex real-time reverse transcription polymerase chain reaction assay for the detection and typing of Vesicular stomatitis virus

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Kate HoleAlfonso Clavijo

Abstract

An improvement to a previously reported real-time reverse transcription polymerase chain reaction (real-time RT-PCR) assay for the detection of Vesicular stomatitis virus (VSV) is described. Results indicate that the new assay is capable of detecting a panel of genetically representative strains of VSV present in North, Central, and South America. The assay is specific for VSV and allows for simultaneous differentiation between Vesicular stomatitis Indiana virus and Vesicular stomatitis New Jersey virus. This real-time RT-PCR is able to detect current circulating strains of VSV and can be used for rapid diagnosis of VSV and differentiation of VSV from other vesicular diseases, such as foot-and-mouth disease.

References

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Feb 14, 2006·Journal of Virological Methods·Thomas Bruun RasmussenMonserrat Agüero
Apr 19, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kate HoleLuis A Pineda
Apr 27, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mariko MoniwaPaul R Kitching
Oct 30, 2007·Journal of Virological Methods·Jovita FernándezJosé Manuel Sánchez-Vizcaíno
May 31, 2008·Archives of Virology·Steven J PauszekLuis L Rodríguez
Jul 16, 2008·Veterinary Microbiology·Philippa J M JackDavid B Boyle
Aug 1, 2008·Transboundary and Emerging Diseases·B Rodriguez-SanchezSándor Belák
Mar 17, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·William C WilsonLuis L Rodríguez

Citations

Jul 14, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Alfonso ClavijoWilliam C Wilson
Apr 30, 2020·Pathogens·Paula Rozo-LopezBarbara S Drolet
May 26, 2020·Archives of Virology·Mariana Lázaro SalesJenner Karlisson Pimenta Dos Reis

Datasets Mentioned

BETA
J02428

Methods Mentioned

BETA
PCR
electrophoresis
enzyme-linked immunosorbent assay
ELISA

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Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
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