Improvement and transcriptome analysis of root architecture by overexpression of Fraxinus pennsylvanica DREB2A transcription factor in Robinia pseudoacacia L. 'Idaho'

Plant Biotechnology Journal
Yu XiuHuafang Wang

Abstract

Transcription factors play a key role to enable plants to cope with abiotic stresses. DREB2 regulates the expression of several stress-inducible genes and constitutes major hubs in the water stress signalling webs. We cloned and characterized a novel gene encoding the FpDREB2A transcription factor from Fraxinus pennsylvanica, and a yeast activity assay confirmed its DRE binding and transcription activation. Overexpression of FpDREB2A in R. pseudoacacia showed enhanced resistance to drought stress. The transgenic plant survival rate was significantly higher than that of WT in soil drying and re-watering treatments. Transgenic lines showed a dramatic change in root architecture, and horizontal and vertical roots were found in transgenic plants compared to WT. The vertical roots penetrated in the field soil to more than 60 cm deep, while horizontal roots expanded within the top 20-30 cm of the soil. A physiological test demonstrated that chlorophyll contents were more gradually reduced and that soluble sugars and proline levels elevated more sharply but malondialdehyde level stayed the same (P < 0.05). Plant hormone levels of abscisic acid and IAA were higher than that of WT, while gibberellins and zeatin riboside were found to be...Continue Reading

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Citations

Feb 9, 2017·Frontiers in Plant Science·Tao WeiYong Zhang
Jul 6, 2020·Plant Molecular Biology·Jing-Bo ZhangXue-Bao Li
Jan 24, 2019·Frontiers in Plant Science·Andrea PolleAntoine Harfouche

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Datasets Mentioned

BETA
DQ778006

Methods Mentioned

BETA
transgenic
PCR
electrophoresis
MDA

Software Mentioned

SOAP novo
MISA
IDEG
TrEMBL
BLAST
Getorf
Trinity
SOAP de novo
IDEG6

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