Improvement in cell-bound phytase activity of Pichia anomala by permeabilization and applicability of permeabilized cells in soymilk dephytinization

Journal of Applied Microbiology
Parvinder Kaur, T Satyanarayana

Abstract

Whole cell permeabilization of Pichia anomala to ameliorate the cell-bound phytase activity and usability of permeabilized cells in dephytinization of soymilk. The cells of P. anomala were subjected to permeabilization using the surfactant Triton X-100 to overcome the permeability barrier and prepare whole cell biocatalysts with high phytase activity. The statistical approach, response surface methodology (RSM) was used to optimize the operating conditions for permeabilization. The treatment of cells with 5% Triton X-100 for 30 min resulted in c. 15% enhancement in cell-bound phytase activity. The shrinkage of protoplast was observed, although cell viability and phytase stability were not significantly altered. The free as well as immobilized permeabilized cells hydrolysed soymilk phytate, and the latter could be reused over four consecutive cycles. Whole cell permeabilization of P. anomala using Triton X-100 led to enhancement in cell-bound phytase activity. The viability and integrity of yeast cells were not significantly affected because of permeabilization. The permeabilized P. anomala cells effectively dephytinized soymilk, and the permeabilized cells immobilized in alginate could be reused because of sustained phytase act...Continue Reading

References

Sep 1, 1977·CRC Critical Reviews in Food Science and Nutrition·D K Salunkhe, M T Wu
Oct 1, 1978·Analytical Biochemistry·G F MiozzariR Hütter
Nov 1, 1977·Archives of Biochemistry and Biophysics·P Schlieper, E De Robertis
Jan 1, 1976·Canadian Journal of Microbiology·V P Choudary, G R Rao
May 2, 1973·European Journal of Biochemistry·R SerranoC Gancedo
Mar 1, 1982·Analytical Biochemistry·H Felix
Aug 1, 1983·Journal of General Microbiology·S P RamM G Shepherd
May 1, 1984·Analytical Biochemistry·P V Choudary
Jul 1, 1997·The Journal of Nutrition·M E BentleyK H Brown
Jun 20, 1998·Poultry Science·D D Maenz, H L Classen
Apr 16, 2003·Critical Reviews in Biotechnology·Ashima Vohra, T Satyanarayana
Oct 11, 2003·Biotechnology Advances·J K Park, H N Chang
Nov 25, 2003·Biochemical and Biophysical Research Communications·Edward J Mullaney, Abul H J Ullah
Jul 30, 2004·Journal of Applied Microbiology·A Vohra, T Satyanarayana
Oct 20, 2005·Journal of Bioscience and Bioengineering·C QuanY Ohta
Jan 16, 2007·Applied Microbiology and Biotechnology·Rachel Ruizhen Chen
Jan 20, 2007·FEMS Yeast Research·Jason van StadenMarinda Viljoen-Bloom
Jun 21, 2007·Critical Reviews in Biotechnology·Parvinder KaurT Satyanarayana

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