Improvement in islet yield from obese donors for human islet transplants

Transplantation
Ippei MatsumotoB J Hering

Abstract

The feasibility of human islet transplantations has been firmly established. To increase the number of islet transplants, the suitability of pancreases from organ donors considered inappropriate for pancreas transplantations must be evaluated. We isolated islets from 114 human cadaver donor pancreases by the automated Ricordi method, followed by purification using continuous-density gradients. We divided the pancreases into two groups by donor body mass index (BMI)--group 1: n=51, BMI of 30 or more; group 2: n=63, BMI of less than 30. We compared the results of human islet isolation, in vitro potency assays, and a nude mouse bioassay. In group 1 (vs. group 2), we found a significantly higher mean pancreas weight (109.5+/-30.7 vs. 90.6+/-24.0 g; P=0.0002); higher mean islet equivalents/pancreas, after digestion (442,565+/-238,741 vs. 289,860+/-158,995; P<0.0001) and after purification (319,129+/-164,002 vs. 215,753+/-126,089; P=0.0002); and a higher islet isolation success rate--defined as isolations yielding more than 300,000 islet equivalents/pancreas, with purities of more than 50% (37.3% [19 of 51 pancreases] vs. 15.9% [10 of 63]; P=0.009). Our in vitro potency assays and bioassay uncovered no differences between the two gro...Continue Reading

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Citations

Jun 20, 2006·Diabetologia·A N BalamuruganR Bottino
Oct 12, 2012·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Sachiyo ShirakawaYonson Ku
Oct 8, 2005·Transplantation·Doug O'GormanJonathan R T Lakey
Jan 2, 2007·Transplantation·Shinichi MatsumotoKoichi Tanaka
Apr 15, 2008·Transplantation·Stephen C HanleyLawrence Rosenberg
Jul 5, 2012·Cold Spring Harbor Perspectives in Medicine·Michael McCall, A M James Shapiro
Jul 6, 2010·The Keio Journal of Medicine·Nobuyo HatanakaMasahiro Kami
Jul 17, 2014·Transplantation·Nadja NiclaussThierry Berney
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Apr 1, 2008·Transplantation Proceedings·Y SakumaH Ichii
Jun 14, 2005·Transplant International : Official Journal of the European Society for Organ Transplantation·Thierry BerneyPhilippe Morel
Aug 12, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·T AnazawaD E R Sutherland
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Jul 26, 2011·Diabetes & Metabolism Journal·Shinichi Matsumoto
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Dec 10, 2009·Cell Transplantation·Shinichi MatsumotoMarlon Levy
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May 3, 2013·Cell Transplantation·Denise E HillingPerla J Marang-van de Mheen
Jan 23, 2019·Islets·Michael P DybalaManami Hara
Feb 25, 2020·Journal of Diabetes·Françoise CastexAnne-Dominique Lajoix
Oct 3, 2018·Cell Transplantation·Elisabet Estil LesEduard Montanya

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