Improvement in organophosphorus hydrolase activity of cell surface-engineered yeast strain using Flo1p anchor system.

Biotechnology Letters
Takeshi FukudaShin-ichiro Suye

Abstract

Organophosphorus hydrolase (OPH) hydrolyzes organophosphorus esters. We constructed the yeast-displayed OPH using Flo1p anchor system. In this system, the N-terminal region of the protein was fused to Flo1p and the fusion protein was displayed on the cell surface. Hydrolytic reactions with paraoxon were carried out during 24 h of incubation of OPH-displaying cells at 30 degrees C. p-Nitrophenol produced in the reaction mixture was detected by HPLC. The strain with highest activity showed 8-fold greater OPH activity compared with cells engineered using glycosylphosphatidylinositol anchor system, and showed 20-fold greater activity than Escherichia coli using the ice nucleation protein anchor system. These results indicate that Flo1p anchor system is suitable for display of OPH in the cell surface-expression systems.

References

Nov 1, 1994·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·K TuovinenO Hänninen
Dec 19, 2000·Enzyme and Microbial Technology·H Shi, Wen Su W
Feb 15, 2001·Biotechnology Progress·M ShimazuW Chen
Jun 12, 2003·Journal of Agricultural and Food Chemistry·Jae Koo LeeBruce D Hammock
Aug 8, 2006·Biotechnology Progress·Katsumi TakayamaAshok Mulchandani
May 12, 2009·Biotechnology Letters·Ken HoriiAkihiko Kondo

❮ Previous
Next ❯

Citations

Jun 2, 2012·Applied Microbiology and Biotechnology·Tsutomu TanakaAkihiko Kondo
Sep 28, 2010·Biotechnology Letters·Kouichi Kuroda, Mitsuyoshi Ueda
Mar 29, 2013·Applied Biochemistry and Biotechnology·Yang SunQuanshun Li
Aug 11, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Katsumi TakayamaMitsuyoshi Ueda
Jun 16, 2016·Bioscience, Biotechnology, and Biochemistry·Mitsuyoshi Ueda
Aug 15, 2015·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Meng LiLixin Ma
Feb 13, 2018·Applied Microbiology and Biotechnology·Cecilia Andreu, Marcel Lí Del Olmo
Jan 1, 2014·Biocontrol Science·Seiji Shibasaki, Mitsuyoshi Ueda
Jan 4, 2021·Bioprocess and Biosystems Engineering·Mengqi YeGuanjun Chen

❮ Previous
Next ❯

Methods Mentioned

BETA
biosensors
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.