Improvement of bioinsecticides production through mutagenesis of Bacillus thuringiensis by u.v. and nitrous acid affecting metabolic pathways and/or delta-endotoxin synthesis

Journal of Applied Microbiology
D GhribiSamir Jaoua

Abstract

The present work aimed to obtain bioinsecticide over-producing mutants through classical mutagenesis of vegetative cells of Bacillus thuringiensis (Bt) by using u.v. and nitrous acid, and to evidence the involvement of cell metabolism in delta-endotoxin synthesis. Vegetative cells of Bt were treated by nitrous acid (0.17 mg ml(-1)) or exposed to u.v. rays (emitted at a wave length of 240 nm). The isolated survivors were screened on the basis of the production of delta-endotoxins and biomass in glucose and/or in gruel-based media at two aeration conditions. Bioinsecticide over-producing mutants were obtained with high frequencies because random mutations were shown to affect cell metabolism at different pathways related to the regulation of delta-endotoxin synthesis. Classical mutagenesis of Bt cells lead to the isolation of a large variety of delta-endotoxin over-producing mutants that could be classified into six groups based on the location of the mutations, particularly in metabolism pathways and delta-endotoxin synthesis. High frequencies of delta-endotoxin over-producing mutants of Bt could be obtained through classical mutagenesis of vegetative cells. This should contribute to a significant reduction of production and uti...Continue Reading

References

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Citations

Jan 22, 2014·International Journal of Biological Macromolecules·Jihen ElleuchSamir Jaoua
Feb 4, 2012·Journal of Economic Entomology·Subbiah Poopathi, S Abidha
Dec 1, 2017·Journal of Medical Entomology·Lingling ZhangXiong Guan
Apr 26, 2020·Environmental Science and Pollution Research International·Patrick AmoateyHameed Sulaiman
Jul 28, 2016·Toxicon : Official Journal of the International Society on Toxinology·Jihen ElleuchRaida Z Zghal

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