Improvement of bread making quality by supplementation with a recombinant xylanase produced by Pichia pastoris

PloS One
Carolina Cândida de Queiroz Brito CunhaCirano José Ulhoa

Abstract

Xylanases (EC 3.2.1.8) are hydrolytic enzymes, which randomly cleave the β-1,4-linked xylose residues from xylan. The synthetic gene xynBS27 from Streptomyces sp. S27 was successfully cloned and expressed in Pichia pastoris. The full-length gene consists of 729 bp and encodes 243 amino acids including 51 residues of a putative signal peptide. This enzyme was purified in two steps and was shown to have a molecular weight of 20 kDa. The purified r-XynBS27 was active against beechwood xylan and oat spelt xylan as expected for GH 11 family. The optimum pH and temperature values for the enzyme were 6.0 and 75 °C, respectively. The Km and Vmax were 12.38 mg/mL and 13.68 μmol min/mg, respectively. The r-XynBS27 showed high xylose tolerance and was inhibited by some metal ions and by SDS. r-XynBS27 was employed as an additive in the bread making process. A decrease in firmness, stiffness and consistency, and improvements in specific volume and reducing sugar content were recorded.

References

Oct 23, 1997·Journal of Biotechnology·P BielyD Kluepfel
Jan 21, 2000·FEMS Microbiology Reviews·J L Cereghino, J M Cregg
Jan 18, 2005·FEMS Microbiology Reviews·Tony CollinsGeorges Feller
Feb 24, 2005·Antonie van Leeuwenhoek·Alan T BullMichael Goodfellow
Jun 10, 2005·Applied Microbiology and Biotechnology·M L T M PolizeliD S Amorim
Oct 4, 2011·Biotechnology Advances·Eda Celik, Pınar Calık
Nov 10, 2011·Biotechnology Advances·Gabriel PaësJohnny Beaugrand
Apr 9, 2013·Applied Biochemistry and Biotechnology·Carolina Cândida de Queiroz Brito-CunhaLuiz Artur Mendes Bataus
Jul 1, 1992·World Journal of Microbiology & Biotechnology·K B Bastawde
Apr 20, 2014·Applied Microbiology and Biotechnology·Mudassar AhmadHelmut Schwab
Feb 18, 2015·Journal of Biotechnology·Sebastian C SpohnerPeter Czermak
Oct 27, 2015·Bioprocess and Biosystems Engineering·Burcu Gündüz Ergün, Pınar Çalık
Jan 1, 2016·3 Biotech·Hillol ChakdarAlok Kumar Srivastava

❮ Previous
Next ❯

Citations

Feb 15, 2020·Journal of Cellular Physiology·Mohsen KarbalaeiHadi Farsiani
Jul 6, 2019·Molecular Biology Reports·Munendra KumarMonisha Khanna Kapur
Jan 22, 2020·Applied Biochemistry and Biotechnology·Thiago Machado PasinMaria de Lourdes Teixeira de Moraes Polizeli
May 27, 2020·Archives of Microbiology·Munendra KumarMonisha Khanna Kapur
Mar 25, 2019·International Journal of Biological Macromolecules·Gabriela Salvador de AmoGustavo Orlando Bonilla-Rodriguez

❮ Previous
Next ❯

Datasets Mentioned

BETA
EU660497

Methods Mentioned

BETA
PCR
gel filtration
enzymatic treatments

Software Mentioned

Statgraphics
Texture Expert

Related Concepts

Related Feeds

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.