Improvement of butanol production in Clostridium acetobutylicum through enhancement of NAD(P)H availability

Journal of Industrial Microbiology & Biotechnology
Feng QiG N Bennett

Abstract

Clostridium acetobutylicum is a natural producer of butanol, butyrate, acetone and ethanol. The pattern of metabolites reflects the partitioning of redox equivalents between hydrogen and carbon metabolites. Here the exogenous genes of ferredoxin-NAD(P)+ oxidoreductase (FdNR) and trans-enoyl-coenzyme reductase (TER) are introduced to three different Clostridium acetobutylicum strains to investigate the distribution of redox equivalents and butanol productivity. The FdNR improves NAD(P)H availability by capturing reducing power from ferredoxin. A butanol production of 9.01 g/L (36.9% higher than the control), and the highest ratios of butanol/acetate (7.02) and C4/C2 (3.17) derived metabolites were obtained in the C acetobutylicum buk- strain expressing FdNR. While the TER functions as an NAD(P)H oxidase, butanol production was decreased in the C. acetobutylicum strains containing TER. The results illustrate that metabolic flux can be significantly changed and directed into butanol or butyrate due to enhancement of NAD(P)H availability by controlling electron flow through the ferredoxin node.

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Citations

Jun 17, 2020·Nature Chemical Biology·Ashty S KarimMichael C Jewett
Aug 28, 2020·Journal of Industrial Microbiology & Biotechnology·SongI HanGeorge N Bennett
Feb 6, 2021·Journal of Agricultural and Food Chemistry·Hongxuan WuFeng Qi
Apr 25, 2021·Nature Communications·Valerie De AndaBrett J Baker

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Methods Mentioned

BETA
genetic modifications
PCR

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