Improvement of combined FISH and immunofluorescence to trace the fate of somatic stem cells after transplantation

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Chiara DonadoniGiacomo P Comi

Abstract

Fluorescence in situ hybridization (FISH) combined with immunohistochemistry of tissue-specific markers provides a reliable method for characterizing the fate of somatic stem cells in transplantation experiments. Furthermore, the association between FISH and fluorescent gene reporter detection can unravel cell fusion phenomena, which could account for apparent transdifferentiation events. However, despite the widespread use of these techniques, they still require labor-extensive protocol adjustments to achieve correct and satisfactory simultaneous signal detection. In the present paper, we describe an improvement of simultaneous FISH and immunofluorescence detection. We applied this protocol to the identification of transplanted human and mouse hematopoietic stem cells in murine brain and muscle. This technique provides unique opportunities for following the path taken by transplanted cells and their differentiation into mature cell types.

References

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Citations

Jun 28, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Xiujin XiaVincent F La Russa
Jul 23, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Rustam R MundegarAnton Wernig
Mar 11, 2005·Expert Opinion on Biological Therapy·Bruce A BunnellCarl F Perez
Feb 28, 2008·Applied Immunohistochemistry & Molecular Morphology : AIMM·Michael BzorekLinda Hansen
Sep 10, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stefania CortiGiacomo P Comi
Jun 10, 2011·Cellular Oncology (Dordrecht)·Timo GaiserKerstin Heselmeyer-Haddad

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